Serotype of Nigerian rotavirus strains
Serotype of Nigerian rotavirus strains
复制标题
尼日利亚轮状病毒株血清型
DOI：
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发表时间：
1997
影响因子：
3.3
通讯作者：
H. Werchau
中科院分区：
文献类型：
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作者：
M. Adah;A. Rohwedder;O. Olaleye;O. Durojaiye;H. Werchau
摘要 采用 PAGE、ELISA、PCR 和斑点杂交技术对 1993 年 12 月至 1995 年 8 月期间从 5 岁以上儿童收集的 314 份粪便样本进行了分析，以了解尼日利亚儿科患者轮状病毒感染的电泳型和血清型分布。 14.3%的儿童轮状病毒抗原呈阳性。 6-9月龄儿童是感染最多的儿童，占所有阳性样本的35.6%。 91.1% 的轮状病毒阳性样本可进行血清分型。未检测到血清型 G2、G4 和 G8。 G3 血清型在尼日利亚南部占主导地位（62.5%），而混合感染特异性在尼日利亚北部更为普遍（63.6%）。一些无法分型的样本的存在可能表明所使用的血清型特异性引物和 cDNA 探针无法检测到的血清型。确定了 26 个（57.7%）阳性样品的电泳型。在短模式和长模式电泳型中分别观察到两种和三种迁移模式。讨论了对该国疫苗开发和使用的影响。
Summary Three hundred and fourteen stool samples collected from children >5 years between December 1993 and August 1995 were analysed by PAGE, ELISA, PCR and Dot‐blot hybridization technique for electropherotype and serotype distribution of rotavirus infection among Nigerian paediatric patients. 14.3% of the children were positive for rotavirus antigen. Children aged 6–9 months were most often infected, accounting for 35.6% of all positive samples. 91.1% of rotavirus‐positive samples could be serotyped. Serotypes G2, G4 and G8 were not detected. Serotype G3 predominated (62.5%) in southern Nigeria, while mixed infection specificity was more widespread (63.6%) in northern Nigeria. The presence of some untypeable samples may indicate serotypes which the serotype‐specific primers and cDNA probes used could not detect. Electropherotypes of 26 (57.7%) of the positive samples were determined. Two and 3 migration patterns were observed among the short and long‐pattern electropherotypes, respectively. Implications for vaccine development and utilization in the country are discussed.