Chinese hamster ovary cell mutants defective in the internalization of ricin.
Chinese hamster ovary cell mutants defective in the internalization of ricin.
复制标题
中国仓鼠卵巢细胞突变体蓖麻毒素内化有缺陷。
DOI：
10.1128/mcb.2.5.535-544.1982
复制
发表时间：
1982
影响因子：
5.3
通讯作者：
Wu,HC
中科院分区：
文献类型：
--
作者：
Ray,B;Wu,HC
Chinese hamster ovary mutants simultaneously resistant to ricin andPseudomonastoxin have been isolated. Two mutant cell lines (4-10 and 11-2) were found to retain normal levels of binding of both ricin andPseudomonastoxin. They were defective in the internalization of [125I]ricin into the mutant cells, as measured by both a biochemical assay for ricin internalization and electron microscopic autoradiographic studies. Although pretreatment of Chinese hamster ovary cells with a Na+/K+ionophore, nigericin, resulted in an enhancement of the cytotoxicities of ricin andPseudomonastoxin in the wild-type Chinese hamster ovary cells, preculture of the mutant cells did not alter the susceptibility of the mutant cells to either toxin. These results provide further evidence that there is a common step in the internalization process for ricin andPseudomonastoxin.
