COMPOSITION OF BOVINE MUSCLE LIPIDS AT VARIOUS CARCASS LOCATIONS
COMPOSITION OF BOVINE MUSCLE LIPIDS AT VARIOUS CARCASS LOCATIONS
复制标题
DOI：
10.1111/j.1365-2621.1968.tb01347.x
复制
发表时间：
1968-01-01
影响因子：
3.9
通讯作者：
STOUFFER, JR
中科院分区：
文献类型：
--
作者：
OKEEFE, PW;WELLINGTON, GH;STOUFFER, JR
从牛半腱肌、肱三头肌和背最长肌中提取肌内脂肪，经油酸柱层析分离成磷脂和中性脂肪。尽管每个部位的总脂肪含量范围很广，但所有三种肌肉中的磷脂含量约为500 mg/100 g肌肉组织。这一结果，再加上与其他两块肌肉相比，半腱肌的总脂肪含量较低，表明与肱三头肌或背最长肌相比，半腱肌的总脂肪中磷脂物质的百分比显著较高。使用极性和非极性色谱柱上获得的保留时间数据，在两种脂质组分中鉴定了脂肪酸。当溴化样品的色谱图上未出现不饱和脂肪酸峰时，确认了不饱和脂肪酸的同一性。有显着更多的C14：0的背最长肌中性脂肪组分比半腱肌中性脂肪组分。在磷脂中，与半腱肌或肱三头肌相比，背最长肌中的C16：0显著更多，C18：0显著更少。虽然这两个脂质组分的背最长肌含有略高百分比的总饱和脂肪酸比相应的馏分中的其他两个肌肉，效果并不显着。
Bovine intramuscular lipids extracted from the semitendinosus, triceps brachii and longissimus dorsi muscles were fractionated into phospholipids and neutral fats by silicic acid column chromatography. In spite of the wide range in total fat content at each location, phospholipids were present in all three muscles at a level of approximately 500 mg per 100 g of muscle tissue. This result, coupled with the lower total fat content of the semitendinosus as compared to the other two muscles, indicated a significantly higher percentage of phospholipid material in the total fat from the semitendinosus as compared to the triceps brachii or longissimus dorsi.The fatty acids were identified in both lipid fractions using retention time data obtained on both a polar and a non‐polar column. The identity of the unsaturated fatty acids was confirmed when their peaks did not appear on the chromatographs obtained from brominated samples. There was significantly more C14:0 in the longissimus dorsi neutral fat fractions than in the semitendinosus neutral fat fractions. In the phospholipids, there was significantly more C16:0 and significantly less C18:0 in the longissimus dorsi as compared to either the semitendinosus or triceps brachii. Although the two lipid fractions of the longissimus dorsi contained slightly higher percentages of total saturated fatty acids than the corresponding fractions in the other two muscles, the effects were not significant.