Effects of Arsenic Trioxide Exposure on Heat Shock Protein Response in the Immune Organs of Chickens
Effects of Arsenic Trioxide Exposure on Heat Shock Protein Response in the Immune Organs of Chickens
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DOI：
10.1007/s12011-015-0389-1
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发表时间：
2016-01-01
影响因子：
3.9
通讯作者：
Xing, Mingwei
中科院分区：
文献类型：
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作者：
Guo, Ying;Zhao, Panpan;Xing, Mingwei
三氧化二砷 (As2O3) 是一种五价砷，最近被发现与免疫功能受损有关。热休克蛋白（Hsps）是一组高度保守的蛋白质，当生物体暴露于各种应激条件时会快速合成。本研究的目的是确定As2O3对鸡免疫器官（脾、胸腺和法氏囊（BF））中热休克蛋白（Hsp90、Hsp70、Hsp60、Hsp40和Hsp27）表达水平的影响。 72只1日龄雄性海兰鸡随机分为4组，即低As组（L组）、中As组（M组）、高As组（H组）和对照组（C组）。收集免疫器官，在第30、60和90天检测Hsp信使RNA（mRNA）和蛋白水平。结果显示，与As2O3处理组相比，Hsp mRNA（Hsp90、Hsp70、Hsp60、Hsp40和Hsp27）和蛋白（Hsp70和Hsp60）表达水平显着增加（p < 0.05或p < 0.01）。与相应的对照组。综上所述，这些结果表明 As2O3 影响免疫器官中热休克蛋白的水平。
Arsenic trioxide (As2O3), a kind of pentavalent arsenic, has recently been linked to disrupted immune function. Heat shock proteins (Hsps), a group of highly conserved proteins, are rapidly synthesised when living organisms are exposed to various stress conditions. The objective of this study is to determine the effects of As2O3 on the expression level of Hsps (Hsp90, Hsp70, Hsp60, Hsp40 and Hsp27) in the immune organs (spleen, thymus and bursa of Fabricius (BF)) of chickens. A total of 72 1-day-old male Hy-line chickens were randomly divided into four groups, including the low-As group (L group), middle-As group (M group), high-As group (H group) and control group (C group). Immune organs were collected, and levels of Hsp messenger RNA (mRNA) and protein were examined on days 30, 60 and 90. The results showed that the levels of Hsp mRNA (Hsp90, Hsp70, Hsp60, Hsp40 and Hsp27) and protein (Hsp70 and Hsp60) expression were significantly increased (p < 0.05 or p < 0.01) in the As2O3 treatment groups compared with the corresponding control groups. Taken together, these results suggest that As2O3 influences the level of Hsps in immune organs.