Effects of gas atmosphere, platelet-derived growth factor and leukemia inhibitory factor on cell numbers of rabbit embryos cultured in a protein-free medium.
Effects of gas atmosphere, platelet-derived growth factor and leukemia inhibitory factor on cell numbers of rabbit embryos cultured in a protein-free medium.
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气氛、血小板源性生长因子和白血病抑制因子对无蛋白培养基中培养的兔胚胎细胞数量的影响。
DOI：
10.1051/rnd:19970110
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Foote,RH
中科院分区：
文献类型：
--
作者：
Giles,JR;Foote,RH
虽然胚胎的培养条件有所改善，但体外发育并不等同于体内生长。将血小板源性生长因子 (PDGF)、小鼠 (mLIF) 或人 (hLIF) 白血病抑制因子和 10% 胎牛血清 (FBS) 添加到无蛋白培养基中，以及两种气体氛围对兔胚胎发育的影响进行评估。向培养基中添加 PDGF、mLIF 或 hLIF 不会导致囊胚总细胞数出现可检测到的差异。与在 5% CO2: 95% 空气下发育的囊胚相比，在 10% CO2: 5% 02: 85% N2 气体气氛下培养兔胚胎导致囊胚总细胞数增加（P<0.01）（分别为 230 vs 159）。添加含 10% FBS S 的 RD（RPMI-1640 和 Dulbecco’s MEM，1:1）培养基提高了总细胞数（264 vs 155）和内细胞团细胞数（71 vs 47）。这些结果表明，当规定的培养条件促进高比例的双细胞胚胎发育成囊胚时，添加某些生长因子并没有产生可检测到的有益效果，而 10% FBS 则改善了培养条件。兔囊胚/内细胞团细胞/氧气/PDGF/LIF
While culture conditions for embryos have improved, development in vitro is not equivalent to in vivo growth. Platelet-derived growth factor (PDGF), mouse (mLIF) or human (hLIF) leukemia inhibitory factor and 10% fetal bovine serum (FBS) added to protein-free culture medium, as well as two gas atmospheres were evaluated for their effect on rabbit embryo development. Adding PDGF, mLIF or hLIF to the culture medium did not result in detectable differences in total cell number for blastocysts. The culture of rabbit embryos under a gas atmosphere of 10% C02: 5% 02: 85% N2 resulted in improved total cell numbers (P< 0.01) for blastocysts compared to those developing under 5% C02: 95% air (230 vs 159, respec-tively). Supplementing RD (RPMI-1640 and Dulbecco’s MEM, 1: 1) medium with 10% FBS S improved the number of total cells (264 vs 155) and inner cell mass cells (71 vs 47). These results indicate that when defined culture conditions promote a high proportion of two-cell embryos developing into blastocysts, the addition of certain growth factors did not have a detectable beneficial effect, while 10% FBS improved culture conditions. rabbit blastocyst/inner cell mass cell/oxygen/PDGF/LIF