Regulation of hepatic microsomal cytochrome P450IIE1 level by dietary lipids and carbohydrates in rats.
Regulation of hepatic microsomal cytochrome P450IIE1 level by dietary lipids and carbohydrates in rats.
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DOI：
10.1093/jn/121.7.959
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发表时间：
1991-07
期刊：
影响因子：
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通讯作者：
Jeong-Sook H. Yoo;S. Ning;C. Pantuck;C. Pantuck;E. Pantuck;E. Pantuck;Chung S. Yang
中科院分区：
文献类型：
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作者：
Jeong-Sook H. Yoo;S. Ning;C. Pantuck;C. Pantuck;E. Pantuck;E. Pantuck;Chung S. Yang
目前的工作测试的假设，即高脂肪/低碳水化合物饮食提高肝微粒体细胞色素P450 IIE 1的水平。雄性Sprague-Dawley大鼠饲喂含有不同比例玉米油/碳水化合物的流质饲料4 d。高脂肪/碳水化合物比饮食喂养的大鼠产生较高的血清丙酮水平和较高的肝微粒体P450 IIE 1含量和N-亚硝基二甲胺脱甲基酶活性比那些低脂肪/碳水化合物比饮食喂养的大鼠。这种膳食脂肪/碳水化合物对P450 IIE 1的影响也在改良的半纯化AIN-76 A饮食中观察到。此外，数量和程度的不饱和膳食脂质影响P450 IIE 1的调节。在中等脂肪水平（5和20%的饮食），大鼠喂养玉米油和鲱鱼油饮食产生较高的P450 IIE 1活性比那些喂养猪油和橄榄油饮食。喂食含有20%玉米油或相当于20%玉米油饮食的亚油酸的饮食的大鼠显示P450 IIE 1水平比喂食无脂肪饮食的大鼠增加了两倍至三倍。喂食25%玉米油饲料的大鼠体内恩氟烷代谢比喂食0.5%玉米油饲料的大鼠高两倍。目前的研究结果表明，组成P450酶水平的饮食脂肪/碳水化合物的比例进行调节。
The present work tests the hypothesis that high fat/low carbohydrate diets elevate the level of liver microsomal cytochrome P450IIE1. Male Sprague-Dawley rats were fed liquid diets containing varied ratios of corn oil/carbohydrate for 4 d. Rats fed diets with higher fat/carbohydrate ratios produced higher serum acetone levels and higher hepatic microsomal P450IIE1 content and N-nitrosodimethylamine demethylase activity than those fed diets with lower fat/carbohydrate ratios. This dietary fat/carbohydrate effect on P450IIE1 also was observed with modified semipurified AIN-76A diets. In addition, both the quantity and the extent of unsaturation of dietary lipids affected P450IIE1 regulation. At moderate fat levels (5 and 20% diet), rats fed corn oil and menhaden oil diets produced higher P450IIE1 activity than those fed lard and olive oil diets. Rats fed a diet containing 20% corn oil or an amount of linoleic acid equivalent to the 20% corn oil diet showed twofold to threefold increases in the level of P450IIE1 over those fed a fat-free diet. Rats fed a 25% corn oil diet showed twofold higher enflurane metabolism in vivo than those fed a 0.5% corn oil diet. The present results suggest that the constitutive P450 enzyme level is regulated by dietary fat/carbohydrate ratios.