Methods and Challenges of Using the Greater Wax Moth (Galleria mellonella) as a Model Organism in Antimicrobial Compound Discovery
Methods and Challenges of Using the Greater Wax Moth (Galleria mellonella) as a Model Organism in Antimicrobial Compound Discovery
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DOI：
10.3390/microorganisms7030085
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发表时间：
2019-03-19
期刊：
影响因子：
4.5
通讯作者：
Jenssen, Havard
中科院分区：
文献类型：
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作者：
Andrea, Athina;Krogfelt, Karen Angeliki;Jenssen, Havard
在非哺乳动物感染模式生物中，大蜡螟Galleria mellonella的幼虫近年来越来越受欢迎。与其他无脊椎动物模型不同，这些幼虫可以在37摄氏度下孵育，并且可以相对精确地给药。尽管越来越多的出版物描述了这种模式生物的使用，但在不同实验室中如何产生模型方面存在很大的差异，包括幼虫大小、年龄、来源、储存和休息期，以及感染和治疗的剂量。在这里，我们就如何处理这些因素提供了建议，以促进不同实验室之间研究的可比性。我们引入了一条线性回归曲线，将幼虫总重量与液体体积相关联，以估计病原体的体内浓度和给药浓度。最后，我们讨论了其他几个方面，包括体内抗生素在幼虫中的稳定性，不同病原体的感染剂量和幼虫选择的建议准则。
Among non-mammalian infection model organisms, the larvae of the greater wax moth Galleria mellonella have seen increasing popularity in recent years. Unlike other invertebrate models, these larvae can be incubated at 37 degrees C and can be dosed relatively precisely. Despite the increasing number of publications describing the use of this model organism, there is a high variability with regard to how the model is produced in different laboratories, with respect to larva size, age, origin, storage, and rest periods, as well as dosing for infection and treatment. Here, we provide suggestions regarding how some of these factors can be approached, to facilitate the comparability of studies between different laboratories. We introduce a linear regression curve correlating the total larva weight to the liquid volume in order to estimate the in vivo concentration of pathogens and the administered drug concentration. Finally, we discuss several other aspects, including in vivo antibiotic stability in larvae, the infection doses for different pathogens and suggest guidelines for larvae selection.