Characterization of exosomal long non-coding RNAs in chondrogenic differentiation of human adipose-derived stem cells
Characterization of exosomal long non-coding RNAs in chondrogenic differentiation of human adipose-derived stem cells
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外泌体长非编码 RNA 在人脂肪干细胞软骨分化中的表征
DOI：
10.1007/s11010-020-04003-2
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发表时间：
2021
影响因子：
4.3
通讯作者：
Shu Hu
中科院分区：
文献类型：
--
作者：
Ziji Zhang;Guangxin Huang;Guping Mao;Shu Hu
来源于软骨干细胞的外泌体和长链非编码RNA（lncRNA）在软骨再生中起关键作用。在这里，我们研究了外泌体lncRNA在人脂肪干细胞（hADSC）软骨形成中的表达谱。体外诱导hADSCs向软骨细胞分化，分离未分化hADSCs和成软骨hADSCs的Exosomes。通过RNA测序分析分离的外泌体中的LncRNA和mRNA表达谱。将所得数据进行基因本体（GO）术语和KEGG通路分析以鉴定差异表达的lncRNA。与来自未分化hADSC的外泌体相比，我们在来自软骨形成hADSC的外泌体中鉴定出23种上调和163种下调的lncRNA。此外，mRNA表达数据分析显示，在软骨形成hADSC的外泌体中有968个上调基因和572个下调基因。通过qRT-PCR进一步验证Lncrna和mRNA表达水平。利用差异表达的lncRNA和mRNA构建编码-非编码基因共表达网络（CNC网络）。GO术语和KEGG途径富集分析揭示了未分化hADSC和软骨形成hADSC之间的几个显著差异调节过程。综上所述，本研究揭示了hADSCs软骨分化过程中exosomal lncRNA的差异表达，为进一步研究hADSCs来源exosomal的软骨修复机制奠定了基础。
The exosomes derived from chondrogenic stem cells and long non-coding RNAs (lncRNAs) play a key role in cartilage regeneration. Here, we investigated the expression profile of exosomal lncRNAs in chondrogenesis of human adipose derived stem cells (hADSCs). hADSCs were induced to differentiate into chondrocytesin vitro.Exosomes from undifferentiated hADSCs and chondrogenic hADSCs were isolated. LncRNA and mRNA expression profiles in the isolated exosomes were analyzed by RNA sequencing. The resultant data were subjected to gene ontology (GO) terms and KEGG pathway analysis to identify differentially expressed lncRNAs. We identified 23 upregulated and 163 downregulated lncRNAs in exosomes derived from chondrogenic hADSCs compared to that in exosomes from undifferentiated hADSCs. In addition, analysis of mRNA expression data revealed 968 upregulated genes and 572 downregulated genes in exosomes of chondrogenic hADSCs. Lncrna and mRNA expression levels were further validated by qRT–PCR. Differentially expressed lncRNAs and mRNAs were utilized to construct a coding–non–coding gene co-expression network (CNC network). GO terms and KEGG pathway enrichment analysis revealed several significant processes differentially regulated between undifferentiated hADSCs and chondrogenic hADSCs. Taken together, this study revealed the differential expression of exosomal lncRNAs of chondrogenic hADSCs and provided a foundation for future study on the cartilage recovery mechanism of exosomes derived from chondrogenic stem cells.