FACULTATIVE POLYPEPTIDE TRANSLOCATION ALLOWS A SINGLE MESSENGER-RNA TO ENCODE THE SECRETED AND CYTOSOLIC FORMS OF PLASMINOGEN ACTIVATORS INHIBITOR-2
FACULTATIVE POLYPEPTIDE TRANSLOCATION ALLOWS A SINGLE MESSENGER-RNA TO ENCODE THE SECRETED AND CYTOSOLIC FORMS OF PLASMINOGEN ACTIVATORS INHIBITOR-2
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DOI：
10.1002/j.1460-2075.1989.tb08489.x
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发表时间：
1989-11-01
期刊：
影响因子：
11.4
通讯作者：
VASSALLI, JD
中科院分区：
文献类型：
--
作者：
BELIN, D;WOHLWEND, A;VASSALLI, JD
人和鼠单核细胞/巨噬细胞合成两种形式的纤溶酶原激活物抑制剂2（派-2）：一种在胞质溶胶中积累，而另一种转运到内质网中，糖基化并分泌。我们在这里表明，一个单一的mRNA编码两种形式的派-2。首先，通过北方印迹杂交和RNA酶保护检测单个派-2 mRNA。其次，派-2 cDNA的转染导致两种形式的派-2的合成。最后，在体外翻译的派-2 cDNA的mRNA转录本的微粒体膜的存在下产生两种拓扑结构不同的形式的派-2。派-2的胞质和分泌形式不是由于使用两个翻译起始位点而产生的，因为它们的合成在人和鼠基因之间保守的序列背景下同时AUG起始。因此，一种多肽在两个拓扑结构不同的细胞区室中的积累可以通过兼性易位来实现。
Two forms of plasminogen activators inhibitor 2 (PAI-2) are synthesized by human and murine monocytes/macrophages: one accumulates in the cytosol, while the other is translocated into the endoplasmic reticulum, glycosylated and secreted. We show here that a single mRNA encodes both forms of PAI-2. Firstly, a single PAI-2 mRNA was detected by Northern blot hybridization and by RNase protection. Secondly, transfection of a PAI-2 cDNA led to the synthesis of both forms of PAI-2. Finally, in vitro translation of a mRNA transcript of the PAI-2 cDNA in the presence of microsomal membranes generated two topologically distinct forms of PAI-2. The cytosolic and secreted forms of PAI-2 do not result from the use of two translation start sites, since their synthesis initiates at the same time AUG, in a sequence context that is conserved between the human and murine genes. Thus, the accumulation of one polypeptide into two topologically distinct cellular compartments can be acheived by facultative translocation.