Ethanol impairs memory of a simple discrimination in adolescent rats at doses that leave adult memory unaffected
Ethanol impairs memory of a simple discrimination in adolescent rats at doses that leave adult memory unaffected
复制标题
DOI：
10.1016/j.nlm.2003.08.005
复制
发表时间：
2004-01-01
影响因子：
2.7
通讯作者：
Spear, NE
中科院分区：
文献类型：
--
作者：
Land, C;Spear, NE
与成年大鼠相比，青春期大鼠对乙醇的低温、抗焦虑、运动障碍、催眠和致命作用不太敏感。然而，体外实验表明，断奶前或青春期大鼠的海马神经活动比成年大鼠更容易受到乙醇的影响。这些数据得到了体内结果的补充，表明预训练乙醇会损害青少年大鼠的学习，但剂量不会影响成年大鼠的学习。为了确定训练后乙醇对青少年记忆的影响是否与成人不同，对两个年龄段的斯普拉格-道利白化大鼠进行了食欲激发的气味辨别训练，要求它们挖掘有香味的沙子以获得加糖的谷物奖励。训练结束后，受试者立即腹腔注射 0、0.5 或 1 g/kg 乙醇（12.6%）。 48 小时后的测试中，受试者接受无诱饵的判别，并测量在 S+ 和 S- 中挖掘所花费的时间。如果训练后进行乙醇训练，青少年（而非成年人）的辨别能力会受损。随后的实验排除了青少年表现受损是由于乙醇引起的条件性味觉或气味厌恶造成的可能性。因此，在食欲调节测试中，相对于成年人，青春期大鼠的记忆力更容易受到乙醇的影响，该测试排除了乙醇对学习过程中感觉和动机影响的影响。 (C) 2003 Elsevier Inc. 保留所有权利。
Adolescent rats are less sensitive than adults to the hypothermic, anxiolytic, motor impairing, hypnotic, and lethal effects of ethanol. In vitro experiments nevertheless suggest that hippocampal neural activity is more affected by ethanol in preweanling or adolescent rats than in adults. These data are complemented by in vivo results showing that pretraining ethanol impairs learning in adolescent rats at doses that do not affect adult learning. In order to determine if posttraining ethanol affects memory differently in adolescents than in adults, Sprague-Dawley albino rats of both ages were trained in an appetitively motivated odor discrimination in which they were required to dig in scented sand for sweetened cereal reward. Immediately after training subjects received intraperitoneal injections of 0, 0.5, or 1 g/kg ethanol (12.6%). At test, 48 h later, subjects were presented with unbaited discriminanda and the time (s) spent digging in the S+ and S- was measured. Adolescents, but not adults, showed impaired discrimination performance if training was followed by ethanol. A subsequent experiment discounted the possibility that impaired adolescent performance was due to ethanol-induced conditioned taste or odor aversions. It thus appears that relative to adults, memory in adolescent rats is more strongly affected by ethanol in a test of appetitive conditioning that excludes ethanol's effects on sensory and motivational influences during the learning experience. (C) 2003 Elsevier Inc. All rights reserved.