Mass spectrometry of N-linked oligosaccharides using atmospheric pressure infrared laser ionization from solution.
Mass spectrometry of N-linked oligosaccharides using atmospheric pressure infrared laser ionization from solution.
复制标题
使用大气压红外激光电离溶液对 N-连接寡糖进行质谱分析。
DOI：
10.1002/jms.669
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Doroshenko,VladimirM
中科院分区：
文献类型：
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作者：
Tan,PhillipV;Taranenko,NelliI;Laiko,VictorV;Yakshin,MikhailA;Prasad,CoorgR;Doroshenko,VladimirM
在四极杆离子阱质谱仪上实施的大气压（AP）红外（IR）激光电离技术用于分析溶液中的未衍生化N-连接寡糖。实验是在大气压溶液红外电离（AP-IRIS）离子源上进行的，该离子源与以前的AP IR基质辅助激光解吸/电离（MALDI）接口不同，因为该离子源在没有提取电场的情况下使用更高功率的2.94 µm IR激光器工作。一般术语“IRIS”被用于电离机制不同于MALDI，并且尚未完全阐明。AP-IRIS离子源对支链寡糖具有飞摩尔级灵敏度。AP-IRIS对低聚甘露糖-6和核心岩藻糖基化聚糖M3 N2 F的灵敏度比在具有2，4，6-三羟基苯乙酮基质的AP UV-MALDI上可获得的最佳结果提高了16倍。IR和UV情况之间的比较还显示，具有保守的三甘露糖基核心的聚糖在IR光谱中的碎片较少，核心被岩藻糖取代。复合、高甘露糖和唾液酸化聚糖的混合物导致每种糖具有分子离子峰的正离子质谱。聚糖混合物中钠化分子离子的串联质谱主要显示糖苷（B，Y）裂解。报道的结果显示了AP-IRIS的实际效用，而电离机制仍在研究中。版权所有© 2004年约翰威利父子有限公司。
An atmospheric pressure (AP) infrared (IR) laser ionization technique, implemented on a quadrupole ion trap mass spectrometer, was used to analyze underivatized, N‐linked oligosaccharides in solution. Experiments were conducted on an atmospheric pressure infrared ionization from solution (AP‐IRIS) ion source which differed from previous AP IR matrix‐assisted laser desorption/ionization (MALDI) interfaces in that the ion source operated in the absence of an extraction electric field with a higher power 2.94 µm IR laser. The general term ‘IRIS’ is used as the mechanism of ionization differs from that of MALDI, and is yet to be fully elucidated. The AP‐IRIS ion source demonstrated femtomole‐level sensitivity for branched oligosaccharides. AP‐IRIS showed ∼ 16 times improved sensitivity for oligomannose‐6 and the core‐fucosylated glycan M3N2F over optimal results obtainable on a AP UV‐MALDI with a 2,4,6‐trihydroxyacetophenone matrix. Comparison between IR and UV cases also showed less fragmentation in the IR spectrum for a glycan with a conserved trimannosyl core, core‐substituted with fucose. A mixture of complex, high‐mannose and sialylated glycans resulted in positive ion mass spectra with molecular ion peaks for each sugar. Tandem mass spectrometry of the sodiated molecular ions in a mixture of glycans revealed primarily glycosidic (B, Y) cleavages. The reported results show the practical utility of AP‐IRIS while the ionization mechanism is still under investigation. Copyright © 2004 John Wiley & Sons, Ltd.