Dietary Selenium Modulates Activation and Differentiation of CD4+ T Cells in Mice through a Mechanism Involving Cellular Free Thiols
Dietary Selenium Modulates Activation and Differentiation of CD4+ T Cells in Mice through a Mechanism Involving Cellular Free Thiols
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DOI：
10.3945/jn.109.120725
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发表时间：
2010-06-01
影响因子：
4.2
通讯作者：
Hoffmann, Peter R.
中科院分区：
文献类型：
--
作者：
Hoffmann, FuKun W.;Hashimoto, Ann C.;Hoffmann, Peter R.
硒（Se）补充剂的免疫增强作用使其成为一种有前途的补充和替代医学模式，以提高免疫力，但硒影响免疫力的机制尚不清楚。用肽/佐剂攻击小鼠，用低硒（0.08 mg/kg）、中硒（0.25 mg/kg）或高硒（1.0 mg/kg）饲料喂养8周。高硒组抗原特异性CD 4（+）T细胞反应较低硒组和中硒组增强。体外CD 4（+）T细胞中的T细胞受体信号随着膳食硒的增加而增加，所有3组在钙动员、氧化爆发、活化T细胞核因子易位和增殖方面彼此不同。与低硒和中硒饲料相比，高硒饲料增加了白细胞介素（IL）-2和IL-2受体高亲和力链的表达。高硒饮食使辅助性T细胞（Th）1/Th 2平衡向Th 1表型倾斜，导致与低硒和中硒饮食相比更高的干扰素-γ和CD 40配体水平。在CF 4（+）T细胞活化之前，各组之间的活性氧水平没有差异，但与中硒和高硒饮食相比，低硒饮食降低了游离巯基。添加外源性游离巯基消除了饮食组之间CD 4（+）T细胞活化的差异。总的来说，这些数据表明，膳食硒水平调节游离巯基水平和CD 4（+）T细胞活化过程中的特定信号事件，影响其增殖和分化。J.营养140：1155-1161，2010.
The immune-enhancing effects of selenium (Se) supplementation make it a promising complementary and alternative medicine modality for boosting immunity, although mechanisms by which Se influences immunity are unclear. Mice fed low (0.08 mg/kg), medium (0.25 mg/kg), or high (1.0 mg/kg) Se diets for 8 wk were challenged with peptide/adjuvant. Antigen-specific CD4(+) T cell responses were increased in the high Se group compared with the low and medium Se groups. T cell receptor signaling in ex vivo CD4(+) T cells increased with increasing dietary Se, with all 3 groups differing from one another in terms of calcium mobilization, oxidative burst, translocation of nuclear factor of activated T cells, and proliferation. The high Se diet increased expression of interleukin (IL)-2 and the high affinity chain of the IL-2 receptor compared with the low and medium Se diets. The high Se diet skewed the T helper (Th)1/Th2 balance toward a Th1 phenotype, leading to higher interferon-gamma and CD40 ligand levels compared with the low and medium Se diets. Prior to CF4(+) T cell activation, levels of reactive oxygen species did not differ among the groups, but the low Se diet decreased free thiols compared with the medium and high Se diets. Addition of exogenous free thiols eliminated differences in CD4(+) T cell activation among the dietary groups. Overall, these data suggest that dietary Se levels modulate free thiol levels and specific signaling events during CD4(+) T cell activation, which influence their proliferation and differentiation. J. Nutr. 140: 1155-1161, 2010.