Invasibility of resident biofilms by allochthonous communities in bioreactors
Invasibility of resident biofilms by allochthonous communities in bioreactors
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DOI：
10.1016/j.watres.2015.05.051
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发表时间：
2015-09-15
期刊：
影响因子：
12.8
通讯作者：
Milferstedt, Kim
中科院分区：
文献类型：
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作者：
Bellucci, Micol;Bernet, Nicolas;Milferstedt, Kim
外来物种的入侵会极大地影响工程生态系统和自然生态系统的群落组成和多样性，包括生物膜。在这项研究中，分子群落指纹图谱方法被用来监测异地联合体在居民多物种生物膜中的建立和定植。为此，接种了自来水或活性污泥的生物膜分别在鼓泡塔反应器W1、W2和S中培养10天，然后暴露在非本地微生物群中。这些联合体包括用于接种自来水的生物膜(W1和W2反应器)的新鲜活性污泥悬浮液和用于活性污泥生物膜的移植的成熟自来水生物膜(S反应器)。在W1和W2中引入原始的、未被占用的优惠券使我们能够进一步研究驻留的生物膜和异地联合体之间对新资源(空间)的竞争。CE-SSCP分析表明，入侵事件发生后，生物膜中优势物种的丰度发生了较大的变化，而其组成变化不大。这表明，生物反应器内的居民群落立即击败了异地微生物，并在短期内塑造了新券和已经定居的表面上的微生物群落组合。然而，随着时间的推移，异地群落的潜在成员可能会长大，影响原始生物膜的多样性和组成。(C)2015爱思唯尔有限公司。保留所有权利。
Invasion of non-native species can drastically affect the community composition and diversity of engineered and natural ecosystems, biofilms included. In this study, a molecular community fingerprinting method was used to monitor the putative establishment and colonization of allochthonous consortia in resident multi-species biofilms. To do this, biofilms inoculated with tap water or activated sludge were grown for 10 days in bubble column reactors W1 and W2, and S, respectively, before being exposed to non-native microbial consortia. These consortia consisted of fresh activated sludge suspensions for the biofilms inoculated with tap water (reactors W1 and W2) and of transplanted mature tap water biofilm for the activated sludge biofilm (reactor S). The introduction of virgin, unoccupied coupons into W1 and W2 enabled us to additionally investigate the competition for new resources (space) among the resident biofilm and the allochthonous consortia. CE-SSCP revealed that after the invasion event changes were mostly observed in the abundance of the dominant species in the native biofilms rather than their composition. This suggests that the resident communities within a bioreactor immediately outcompete the allochthonous microbes and shape the microbial community assemblage on both new coupons and already colonized surfaces for the short term. However, with time, latent members of the allochthonous community might grow up affecting the diversity and composition of the original biofilms. (C) 2015 Elsevier Ltd. All rights reserved.