The phylogeny of Turnip mosaic virus;: comparisons of 38 genomic sequences reveal a Eurasian origin and a recent 'emergence' in east Asia
The phylogeny of Turnip mosaic virus;: comparisons of 38 genomic sequences reveal a Eurasian origin and a recent 'emergence' in east Asia
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DOI：
10.1046/j.1365-294x.2003.01881.x
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发表时间：
2003-08-01
影响因子：
4.9
通讯作者：
Ohshima, K
中科院分区：
文献类型：
--
作者：
Tomimura, K;Gibbs, AJ;Ohshima, K
一个代表性的世界范围内收集的32芜菁花叶病毒（TuMV）分离株的基因组进行了测序，这些，连同6个以前报道的序列，进行了分析。至少五分之一的序列是重组的。在系统发育分析中，使用日本山药花叶病毒的基因组序列作为外群，TuMV序列，没有表现出明确的重组形成了一个单系组，有四个良好的支持谱系。这些分组相关的致病性和种源的差异，所有其他的姐妹组是欧亚B-株分离nonbrassicas，可能代表祖先TuMV人口，和最近出现的“分支的人口可能是BR-株分离株只在东亚发现。8个分离株，都来自东亚，明确的重组，可能是最近的重组事件的后代，而类似的数字，从世界其他地区，似乎是旧的重组。这种差异表明，在亚群中存在明确的重组体可能是最近“出现”的分子特征。
The genomes of a representative world-wide collection of 32 Turnip mosaic virus (TuMV) isolates were sequenced and these, together with six previously reported sequences, were analysed. At least one-fifth of the sequences were recombinant. In phylogenetic analyses, using genomic sequences of Japanese yam mosaic virus as an outgroup, the TuMV sequences that did not show clear recombination formed a monophyletic group with four well-supported lineages. These groupings correlated with differences in pathogenicity and provenance; the sister group to all others was of Eurasian B-strain isolates from nonbrassicas, and probably represents the ancestral TuMV population, and the most recently 'emerged' branch of the population was probably that of the BR-strain isolates found only in east Asia. Eight isolates, all from east Asia, were clear recombinants, probably the progeny of recent recombination events, whereas a similar number, from other parts of the world, were seemingly older recombinants. This difference indicates that the presence of clear recombinants in a subpopulation may be a molecular signature of a recent 'emergence'.