Halolamina rubra sp nov., a haloarchaeon isolated from non-purified solar salt
Halolamina rubra sp nov., a haloarchaeon isolated from non-purified solar salt
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DOI：
10.1007/s10482-014-0145-0
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发表时间：
2014-05-01
影响因子：
2.6
通讯作者：
Nam, Young-Do
中科院分区：
文献类型：
--
作者：
Cha, In-Tae;Yim, Kyung June;Nam, Young-Do
从未纯化的太阳盐中分离到革兰氏染色阴性的棒状极端亲盐菌CBA1107(T)和CBA1108。系统发育分析表明，菌株CBA1107(T)和CBA1108均属于盐藻属，菌株CBA1107(T)与上层盐藻TBN21(T)、盐藻WSY15-H3(T)和盐藻WSY15-H1(T)的16S rRNA基因序列相似性分别为98.3%、97.6%和97.3%；相同菌株间rpoB’基因序列的相似性分别为96.0、95.3和94.6%。两株菌落在培养基上均呈红色。菌株CBA1107(T)在20 ~ 50℃、15 ~ 30% NaCl、6.0 ~ 9.0 pH、0.005 ~ 0.5 M Mg2+条件下生长。这两种菌株的细胞在蒸馏水中溶解。DNA-DNA杂交实验表明，菌株CBA1107(T)与CBA1108亲缘性为97%，与H. pelagica JCM 16809(T)、H. salina JCM 18549(T)和H. salifodinae JCM 18548(T)亲缘性< 50%。测定菌株CBA1107(T)基因组DNA G+C含量为65.1%。两株菌株的主要极性脂质为磷脂酰甘油、磷脂酰甘油磷酸甲酯、磷脂酰甘油硫酸酯和糖脂质为硫酸甘露糖基葡萄糖醚和甘露糖基葡萄糖醚。基于多相分类分析，认为该菌株代表了一个新的分类单元，并被命名为Halolamina rubra sp. nov.，其类型菌株为CBA1107(T) (=CECT 8421（T) =JCM 19436(T)）。
Two Gram-stain negative, rod-shaped and motile extreme halophiles, designated CBA1107(T) and CBA1108, were isolated from non-purified solar salt. Based on the phylogenetic analysis, strains CBA1107(T) and CBA1108 were shown to belong to the genus Halolamina, with similarities for the 16S rRNA gene sequences between strains CBA1107(T) and Halolamina pelagica TBN21(T) , Halolamina salina WSY15-H3(T) and Halolamina salifodinae WSY15-H1(T) of 98.3, 97.6 and 97.3 %, respectively; the similarities for the rpoB' gene sequences between the same strains were 96.0, 95.3 and 94.6 %, respectively. The colonies of both strains were observed to be red pigmented on growth medium. Strain CBA1107(T) was observed to grow at 20-50 A degrees C, in the presence of 15-30 % NaCl, at pH 6.0-9.0, and with 0.005-0.5 M Mg2+. The cells of both strains lysed in distilled water. The DNA-DNA hybridization experiments showed that strain CBA1107(T) shared 97 % relatedness with CBA1108 and < 50 % relatedness with H. pelagica JCM 16809(T), H. salina JCM 18549(T) and H. salifodinae JCM 18548(T). The genomic DNA G+C content of strain CBA1107(T) was determined to be 65.1 mol%. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and glycolipids including sulfated mannosyl glucosyl diether and mannosyl glucosyl diether. Based on the polyphasic taxonomic analyses, the strains are considered to represent a new taxon for which the name Halolamina rubra sp. nov. is proposed, with the type strain CBA1107(T) (=CECT 8421(T) =JCM 19436(T)).