Oxidative stress induced by cerium oxide nanoparticles in cultured BEAS-2B cells
Oxidative stress induced by cerium oxide nanoparticles in cultured BEAS-2B cells
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DOI：
10.1016/j.tox.2007.12.022
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发表时间：
2008-03-12
期刊：
影响因子：
4.5
通讯作者：
Park, Kwangsik
中科院分区：
文献类型：
--
作者：
Park, Eun-Jung;Choi, Jinhee;Park, Kwangsik
采用超临界合成法制备了不同粒径（15、25、30、45 nm）的氧化铈纳米粒子，并利用培养的人肺上皮细胞（BEAS-2B）评价了氧化铈纳米粒子的细胞毒性。暴露的培养细胞的纳米颗粒（5，10，20，40 μ g/ml）导致细胞死亡，ROS增加，GSH减少，和诱导的氧化应激相关基因，如血红素加氧酶-1，过氧化氢酶，谷胱甘肽S-转移酶，硫氧还蛋白还原酶。氧化铈纳米颗粒增加的ROS触发了细胞溶质caspase-3的激活和染色质凝聚，这意味着氧化铈纳米颗粒通过凋亡过程发挥细胞毒性。还测试了纳米颗粒对培养细胞的摄取。研究发现，氧化铈纳米颗粒以聚集颗粒的形式进入细胞质并位于细胞核周围，可诱导纳米颗粒与细胞分子直接相互作用，引起细胞不良反应。(C)2007爱思唯尔爱尔兰有限公司保留所有权利。
Cerium oxide nanoparticles of different sizes (15, 25, 30, 45 nm) were prepared by the supercritical synthesis method, and cytotoxicity was evaluated using cultured human lung epithelial cells (BEAS-2B). Exposure of the cultured cells to nanoparticles (5, 10, 20, 40 mu g/ml) led to cell death, ROS increase, GSH decrease, and the inductions of oxidative stress-related genes such as heme oxygenase-1, catalase, glutathione S-transferase, and thioredoxin reductase. The increased ROS by cerium oxide nanoparticles triggered the activation of cytosolic caspase-3 and chromatin condensation, which means that cerium oxide nanoparticles exert cytotoxicity by an apoptotic process. Uptake of the nanoparticles to the cultured cells was also tested. It was observed that cerium oxide nanoparticles penetrated into the cytoplasm and located in the peri-region of the nucleus as aggregated particles, which may induce the direct interaction between nanoparticles and cellular molecules to cause adverse cellular responses. (C) 2007 Elsevier Ireland Ltd. All rights reserved.