Above pile-up fluorescence microscopy with a 32 Mc/s single-channel time-resolved SPAD system
Above pile-up fluorescence microscopy with a 32 Mc/s single-channel time-resolved SPAD system
复制标题
DOI：
10.1364/ol.444815
复制
发表时间：
2022-01-01
期刊：
影响因子：
3.6
通讯作者：
Rech, Ivan
中科院分区：
文献类型：
--
作者：
Farina, Serena;Labanca, Ivan;Rech, Ivan
时间相关单光子计数（TCSPC）的主要缺点之一通常由堆积失真表示，堆积失真强烈地将最大采集速度限制在激光激发速率的百分之几。基于以前的理论分析，最近，我们提出了第一个，尽我们所知，低失真和高速TCSPC系统能够克服堆积的限制，通过完美匹配的单光子雪崩二极管（SPAD）死时间的激光周期。在这项工作中，我们通过比较实验数据与参考理论框架，在标准荧光测量中验证了所提出的系统。结果，在单通道系统中实现了32 Mc/s的计数率，但仍观察到可忽略的寿命失真。(C)2021光学出版集团
One of the major drawbacks of time-correlated single-photon counting (TCSPC) is generally represented by pile-up distortion, which strongly bounds the maximum acquisition speed to a few percent of the laser excitation rate. Based on a previous theoretical analysis, recently we presented the first, to the best of our knowledge, low-distortion and high-speed TCSPC system capable of overcoming the pile-up limitation by perfectly matching the single-photon avalanche diode (SPAD) dead time to the laser period. In this work, we validate the proposed system in a standard fluorescence measurement by comparing experimental data with the reference theoretical framework. As a result, a count rate of 32 Mc/s was achieved with a single-channel system still observing a negligible lifetime distortion. (C) 2021 Optica Publishing Group