Use of an osmotically sensitive mutant of Propionibacterium freudenreichii subspp. shermanii for the simultaneous productions of organic acids and trehalose from biodiesel waste based crude glycerol
Use of an osmotically sensitive mutant of Propionibacterium freudenreichii subspp. shermanii for the simultaneous productions of organic acids and trehalose from biodiesel waste based crude glycerol
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DOI：
10.1016/j.biortech.2012.01.039
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发表时间：
2012-04-01
影响因子：
11.4
通讯作者：
Choudhury, Bijan
中科院分区：
文献类型：
--
作者：
Ruhal, Rohit;Choudhury, Bijan
最近报道了使用费氏丙酸杆菌亚种生产海藻糖和丙酸的粗甘油的适用性。shermanii的结果表明，粗甘油中KCl的存在可能是粗甘油培养基中海藻糖积累量较高的原因。为了进一步提高海藻糖的产量，该菌株的渗透敏感突变体（在3%NaCl培养基中不能存活）具有较高的海藻糖产量。在突变体中，当使用粗甘油作为碳源时，与亲本菌株相比，相对于生物质和消耗的底物（391 mg/g生物质，90 mg/g消耗的底物）实现的海藻糖产率分别高3倍和4倍。获得的其它主要发酵产物是丙酸（0.42g/g消耗的底物）和乳酸（0.3g/g消耗的底物）。突变体中ADP-葡萄糖焦磷酸化酶活性的提高可能是海藻糖积累增加的原因。(C)2012爱思唯尔有限公司保留所有权利。
Recently suitability of crude glycerol for trehalose and propionic acid productions was reported using Propionibacterium freudenreichii subspp. shermanii and it was concluded that presence of KCl in crude glycerol was the probable reason for higher trehalose accumulation with crude glycerol medium. To further improve trehalose production, an osmotic sensitive mutant of this strain (non-viable in medium with 3% NaCl) with higher trehalose yield was isolated. In mutant, trehalose yields achieved with respect to biomass and substrate consumed (391 mg/g of biomass, 90 mg/g of substrate consumed) were three and four times higher, respectively as compared to parent strain when crude glycerol was used as a carbon source. Other major fermentation products obtained were propionic acid (0.42 g/g of substrate consumed) and lactic acid (0.3 g/g of substrate consumed). It was also observed that in mutant higher activity of ADP-glucose pyrophosphorylase was probably responsible for higher trehalose accumulation. (C) 2012 Elsevier Ltd. All rights reserved.