Fis Regulates Type III Secretion System by Influencing the Transcription of exsA in Pseudomonas aeruginosa Strain PA14.
Fis Regulates Type III Secretion System by Influencing the Transcription of exsA in Pseudomonas aeruginosa Strain PA14.
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Fis 通过影响铜绿假单胞菌 PA14 菌株中 exsA 的转录来调节 III 型分泌系统
DOI：
10.3389/fmicb.2017.00669
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发表时间：
2017
影响因子：
5.2
通讯作者：
Wu W
中科院分区：
文献类型：
--
作者：
Deng X;Li M;Pan X;Zheng R;Liu C;Chen F;Liu X;Cheng Z;Jin S;Wu W
FIS是细菌中一种多功能的DNA结合蛋白。已经在多种细菌中证明了FIS在调节细菌毒力因子和优化细菌对各种环境的适应方面发挥着至关重要的作用。然而，FIS在铜绿假单胞菌毒力和基因调控中的作用仍然很大程度上是未知的。在这里，我们发现在小鼠急性肺炎模型中，FIS是铜绿假单胞菌毒力所必需的。转录组分析表明，在一个fis：：Tn突变体中，T3SS基因，包括主调控子ExsA的表达存在缺陷。我们进一步证明，由exsC启动子驱动的exsC、exsE、exsB和exsA的持续转录是激活T3SS所必需的。FIS被发现与exsB-exsA基因间隔区特异结合，并在exsB到exsA的转录延伸中发挥重要作用。因此，我们发现了FIS在exsA表达调控中的新作用。
Fis is a versatile DNA binding protein in bacteria. It has been demonstrated in multiple bacteria that Fis plays crucial roles in regulating bacterial virulence factors and optimizing bacterial adaptation to various environments. However, the role of Fis in Pseudomonas aeruginosa virulence as well as gene regulation remains largely unknown. Here, we found that Fis was required for the virulence of P. aeruginosa in a murine acute pneumonia model. Transcriptome analysis revealed that expression of T3SS genes, including master regulator ExsA, was defective in a fis::Tn mutant. We further demonstrate that the continuous transcription of exsC, exsE, exsB, and exsA driven by the exsC promoter was required for the activation of T3SS. Fis was found to specifically bind to the exsB-exsA intergenic region and plays an essential role in the transcription elongation from exsB to exsA. Therefore, we found a novel role of Fis in the regulation of exsA expression.