Catalytic RNA reactions of yeast tRNA(Phe) fragments.
Catalytic RNA reactions of yeast tRNA(Phe) fragments.
复制标题
酵母 tRNA(Phe) 片段的催化 RNA 反应。
DOI：
10.1021/bi00158a015
复制
发表时间：
1992
期刊：
影响因子：
2.9
通讯作者：
Termini,J
中科院分区：
文献类型：
--
作者：
Deng,HY;Termini,J
1992年6月25日摘要：我们描述了12种新的催化RNA反应，它们是众所周知的分子内Pb 2+促进的酵母tRNAphe水解的分子间变体。具有T茎环的天然酵母tRNAphe衍生的片段可以作为催化剂，用于含有D茎环的片段在pi 8处的位点特异性水解。最初的报道描述了由天然tRNA衍生的3 '半分子催化切割对应于tRNAPhe的5'半的未修饰的T7转录物。[Sampson，J.R.，Sullivan，F. X.，贝伦湖美国，DiRenzo，A. B.，& Uhlenbeck，OC（1987）Cold SpringHarbor Symp.定量52，267-275]。我们已经研究了反式反应进一步创建一个家庭的底物和催化剂RNA分子的解剖的天然tRNAphe使用化学和酶的方法相结合。使用组合方法与可用的含有T和D茎环的片段进行反式切割活性的搜索。发现12种组合是催化的，并且报告了每种组合的初始速率、fccat和Km。反应的fccat值相差~
Revised Manuscript Received June 25, 1992 abstract: We describe 12 new catalytic RNA reactions which are intermolecular variants of the well-known intramolecular Pb2+-promoted hydrolysis of yeast tRNAphe. Fragments derived from the native yeast tRNAphe which possess the T stem-loop can function as catalysts for the site-specific hydrolysis at pi 8 of D stem-loop-containing fragments. Aninitial report described the catalytic cleavage of an unmodified T7 transcript corresponding to the 5'half of tRNAPhe by a 3'half-molecule derived from the native tRNA.[Sampson, J. R., Sullivan, F. X., Behlen, L. S., DiRenzo, A. B., & Uhlenbeck, OC (1987) Cold SpringHarbor Symp. Quant. Biol. 52, 267-275]. We have investigated the trans reaction further by creating a family of substrate and catalyst RNA molecules by dissection of the native tRNAphe using a combination of chemical and enzymatic methods. A search for cleavage activity in trans was conducted using a combinatorial approach with the available T and D stem-loop-containing fragments. Twelve combinations were found to be catalytic, and initial rates, fccat’s, and Km's are reported for each. The fccat’s for thereactions differ by~