MS5 Mediates Early Meiotic Progression and Its Natural Variants May Have Applications for Hybrid Production in Brassica napus
MS5 Mediates Early Meiotic Progression and Its Natural Variants May Have Applications for Hybrid Production in Brassica napus
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MS5 介导早期减数分裂进程，其天然变体可能可用于甘蓝型油菜的杂交生产
DOI：
10.1105/tpc.15.01018
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发表时间：
2016-06-01
期刊：
影响因子：
11.6
通讯作者：
Cheng, Zhukuan
中科院分区：
文献类型：
--
作者：
Xin, Qiang;Shen, Yi;Cheng, Zhukuan
在减数分裂前期I，染色质经历动态变化，为基本的减数分裂事件建立结构基础。然而，植物染色体结构和减数分裂进程的协调机制仍然知之甚少。本研究对甘蓝型油菜（Brassica napus）中的一个自发不育突变体MS 5（B）MS 5（B）进行了鉴定，发现其减数分裂染色体停滞在细线期。MS5在生殖器官中优先表达，编码一种拟南芥特异性蛋白，携带保守的卷曲螺旋和DUF626结构域，功能未知。MS5是减数分裂中同源物配对所必需的，但不是启动DNA双链断裂所必需的。轴元件相关蛋白ASY1的分布独立于MS5发生，但减数分裂内聚亚基SYN1的定位需要功能性MS5。此外，在MS 5（B）MS 5（B）突变体中，联会复合体的中心元件和重组元件都不能正确形成。研究结果表明，MS5基因参与减数分裂前期早期的减数分裂进程，其等位变异导致育性差异，这可能为杂种优势育种的授粉控制提供一种有前途的策略。
During meiotic prophase I, chromatin undergoes dynamic changes to establish a structural basis for essential meiotic events. However, the mechanism that coordinates chromosome structure and meiotic progression remains poorly understood in plants. Here, we characterized a spontaneous sterile mutant MS5(b)MS5(b) in oilseed rape (Brassica napus) and found its meiotic chromosomes were arrested at leptotene. MS5 is preferentially expressed in reproductive organs and encodes a Brassica-specific protein carrying conserved coiled-coil and DUF626 domains with unknown function. MS5 is essential for pairing of homologs in meiosis, but not necessary for the initiation of DNA double-strand breaks. The distribution of the axis element-associated protein ASY1 occurs independently of MS5, but localization of the meiotic cohesion subunit SYN1 requires functional MS5. Furthermore, both the central element of the synaptonemal complex and the recombination element do not properly form in MS5(b)MS5(b) mutants. Our results demonstrate that MS5 participates in progression of meiosis during early prophase I and its allelic variants lead to differences in fertility, which may provide a promising strategy for pollination control for heterosis breeding.