Design considerations for high speed quantitative mass spectrometry with MALDI ionization
Design considerations for high speed quantitative mass spectrometry with MALDI ionization
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DOI：
10.1016/j.jasms.2006.04.026
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发表时间：
2006-08-01
影响因子：
3.2
通讯作者：
Covey, Thomas R.
中科院分区：
文献类型：
--
作者：
Corr, Jay J.;Kovarik, Peter;Covey, Thomas R.
本文描述了一种用于获得药物和其他低分子量化合物的高速定量测量的三重四极杆质谱仪上的MALDI离子源。特别注意离子的产生和传输现象，影响分析速度，吞吐量，和实际仪器的稳健性。在这方面的参数，影响解吸速度，光束扩散，离子飞行时间，灵敏度，信噪比，离子碎片，样品携带和仪器污染进行了检查和实验结果提供。对MALDI和电喷雾的灵敏度进行了比较，提供了一个实用的参考框架。
A MALDI ion source on a triple quadrupole mass spectrometer constructed for the purpose of obtaining high speed quantitative measurements on drugs and other low molecular weight compounds is described. Particular attention is given to the ion generation and transport phenomena that affect analysis speed, throughput, and practical instrument robustness. In this regard parameters that affect desorption speed, beam spreading, ion flight times, sensitivity, signal-to-noise, ion fragmentation, sample carry-over, and instrument contamination are examined and experimental results are provided. MALDI and electrospray sensitivity is compared, to provide a practical frame of reference.