Day light quality affects the night-break response in the short-day plant chrysanthemum, suggesting differential phytochrome-mediated regulation of flowering
Day light quality affects the night-break response in the short-day plant chrysanthemum, suggesting differential phytochrome-mediated regulation of flowering
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DOI：
10.1016/j.jplph.2012.07.003
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发表时间：
2012-12-01
影响因子：
4.3
通讯作者：
Hisamatsu, Tamotsu
中科院分区：
文献类型：
--
作者：
Higuchi, Yohei;Sumitomo, Katsuhiko;Hisamatsu, Tamotsu
菊花（Chrysanthemum morifolium）是一种短日照植物，当夜间长度超过临界最小值时开花。开花被有效地抑制时，所需的长夜阶段是由一个短暂的暴露于红光（夜休息; NB）中断。随后暴露于远红光（FR）的逆转这种抑制表明参与光敏色素在开花反应。在这里，我们阐明了光质量的作用，在光周期调节菊花开花，通过应用一系列不同的条件。在短日照条件下，白色光（W-SD）、单色红光（R-SD）或单色蓝光（B-SD）下均观察到开花。对于W-SD，单色红光NB（NB-R）对开花的抑制作用最强，而单色蓝光NB（NB-B）和远红光NB（NB-FR）对开花的抑制作用很小。而对于B-SD，NB-B和NB-FR对开花有强烈的抑制作用，但在B-SD中添加单色红光（B + R-SD）时，NB-B和NB-FR对开花没有抑制作用。此外，NB-B的抑制作用后B-SD部分逆转随后暴露于FR光脉冲。B-SD/NB-B（不开花）和B + R-SD/NB-B（开花）条件对生物钟相关基因表达的影响相似。然而，只有前者的组合抑制表达的菊花直向同源物的FLOWERLOCUS T（CmFTL 3）。我们的研究结果表明，参与至少2个不同的光敏色素响应菊花的开花反应。此外，似乎在每日光周期期间提供的光质量影响有效NB所需的光质量。(C)2012 Elsevier GmbH. All rights reserved.
Chrysanthemum (Chrysanthemum morifolium) is a short-day plant, which flowers when the night length is longer than a critical minimum. Flowering is effectively inhibited when the required long-night phase is interrupted by a short period of exposure to red light (night break; NB). The reversal of this inhibition by subsequent exposure to far-red (FR) light indicates the involvement of phytochromes in the flowering response. Here, we elucidated the role of light quality in photoperiodic regulation of chrysanthemum flowering, by applying a range of different conditions. Flowering was consistently observed under short days with white light (W-SD), SD with monochromatic red light (R-SD), or SD with monochromatic blue light (B-SD). For W-SD, NB with monochromatic red light (NB-R) was most effective in inhibiting flowering, while NB with monochromatic blue light (NB-B) and NB with far-red light (NB-FR) caused little inhibition. In contrast, for B-SD, flowering was strongly inhibited by NB-B and NB-FR. However, when B-SD was supplemented with monochromatic red light (B + R-SD), no inhibition by NB-B and NB-FR was observed. Furthermore, the inhibitory effect of NB-B following B-SD was partially reversed by subsequent exposure to a FR light pulse. The conditions B-SD/NB-B (no flowering) and B + R-SD/NB-B (flowering) similarly affected the expression of circadian clock-related genes. However, only the former combination suppressed expression of the chrysanthemum orthologue of FLOWERING LOCUS T (CmFTL3). Our results suggest the involvement of at least 2 distinct phytochrome responses in the flowering response of chrysanthemum. Furthermore, it appears that the light quality supplied during the daily photoperiod affects the light quality required for effective NB. (C) 2012 Elsevier GmbH. All rights reserved.