Simultaneous heterotrophic nitrification and aerobic denitrification of wastewater in granular reactor: Microbial composition by next generation sequencing analysis
Simultaneous heterotrophic nitrification and aerobic denitrification of wastewater in granular reactor: Microbial composition by next generation sequencing analysis
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DOI：
10.1016/j.jwpe.2020.101254
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发表时间：
2020-08-01
影响因子：
7
通讯作者：
Caravelli, Alejandro
中科院分区：
文献类型：
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作者：
Bucci, Paula;Coppotelli, Bibiana;Caravelli, Alejandro
乳品废水含有有限的氮，可用于生物处理。在本研究中，奶酪乳清废水补充氨，以获得合适的COD：N比。在好氧颗粒序批式反应器（SBR）中，研究了有机负荷和微量营养元素对生物脱氮过程的影响。较好的硝化活性（5.04 mg N.（L.h）（-1））的最佳投加量为COD：N = 100：12。在此条件下，同步硝化反硝化导致较高的无机氮去除率。好氧反硝化是由细胞内糖原驱动的。在没有微量营养素的情况下，硝化作用发生时，有机负荷降低（COD：N = 100：22）。通过下一代测序技术分析微生物群落。硝化作用是由异养硝化菌引起的。属于Niabella和Diaphorophylla属和浮游菌目的细菌是负责硝化过程的主要候选者。乳制品废水和富铵废水的共处理是乳制品废水生物处理的一个有前途的策略。
Dairy effluents contain limited nitrogen for biological treatment. In the present study a cheese whey wastewater was supplemented with ammonia to obtain suitable COD:N ratios. The effects of the organic load and micronutrients on the biological nitrogen removal process were analyzed in aerobic granular sequential batch reactors (SBRs). Better nitrifying activity (5.04 mg N.(L.h)(-1)) was achieved at COD:N = 100:12, when micronutrients were added. In this condition, simultaneous nitrification-denitrification led to a higher inorganic nitrogen removal. Aerobic denitrification was driven by intracellular glycogen. In absence of micronutrients, nitrification took place when the organic load was decreased (COD:N = 100:22). The microbial community was analyzed by next-generation sequencing technology. Nitrification was attributed to heterotrophic nitrifiers. Bacteria belonging to Niabella and Diaphorobacter genera and to Planctomycetales order are the main candidates responsible for the nitrification process. Co-treatment of dairy and ammonium-rich wastewater constitutes a promise strategy for the biological treatment of dairy wastewaters.