The separation of vanillate O-demethylase from protocatechuate 3,4-oxygenase by ultracentrifugation.
The separation of vanillate O-demethylase from protocatechuate 3,4-oxygenase by ultracentrifugation.
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通过超速离心将香草酸 O-脱甲基酶与原儿茶酸 3,4-加氧酶分离。
DOI：
10.1042/bj1050767
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发表时间：
1967
期刊：
影响因子：
--
通讯作者：
J. Buswell
中科院分区：
文献类型：
--
作者：
N. J. Cartwright;J. Buswell
1.原儿茶酸3，4-加氧酶在荧光假单胞菌（菌株T）的无细胞提取物的可溶性部分沉淀更迅速地比香草酸O-脱甲基酶在特定条件下，在制备ultracentrance。2.来自该过程的上清液含有香草酸O-脱甲基酶和甲醛脱氢酶，并且当补充有NADH时，氧化香草酸的摄取量为1摩尔氧/摩尔底物和原儿茶酸的积累。3.在氨基脲作为甲醛捕获剂的存在下，这种吸收降低到0.5mol/mol底物。4.提出了香草酸脱甲基过程为氧化脱甲基的原因。
1. Protocatechuate 3,4-oxygenase in the soluble part of a cell-free extract of Pseudomonas fluorescens (strain T) sedimented more rapidly than vanillate O-demethylase under specified conditions in a preparative ultracentrifuge. 2. The supernatant from this process contained vanillate O-demethylase and formaldehyde dehydrogenase, and when supplemented with NADH oxidized vanillate with an uptake of 1 mole of oxygen/mole of substrate and accumulation of protocatechuate. 3. This uptake was decreased to 0.5mole/mole of substrate in the presence of semicarbazide as trapping agent for formaldehyde. 4. Reasons are presented for the process of methyl group removal from vanillate being oxidative demethylation.