The influence of pH and concentration on the zeta potential, hydrophobicity of OVT and the relationship between its structure and interfacial behaviors
The influence of pH and concentration on the zeta potential, hydrophobicity of OVT and the relationship between its structure and interfacial behaviors
复制标题
pH和浓度对OVT Zeta电位、疏水性的影响及其结构与界面行为的关系
DOI：
10.1080/01932691.2021.1880431
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发表时间：
2021-01-29
影响因子：
2.2
通讯作者：
Yang, Wuying
中科院分区：
文献类型：
--
作者：
Hu, Linfang;Wu, Leiyan;Yang, Wuying
蛋白质的结构与其界面行为密切相关，受环境条件的影响很大。本研究旨在探讨pH值对卵转铁蛋白（OVT）粒径、zeta电位、表面疏水性和-SH的影响，并比较不同pH值下OVT的界面行为。结果表明，在3 ~ 13的pH范围内，zeta电位从+10.24 mV变化到-18.83 mV。颗粒大小先增大后减小。当pH值偏离6.5时，-SH暴露量增加，OVT的疏水性增强。与其他pH值下的OVT相比，在pH为5时，OVT具有更高的穿透率和重排率，从而在界面处更快地形成高弹性蛋白膜（膨胀黏性模量为46.32，膨胀弹性模量为16.33 mN/m）。高弹性界面吸附层可以防止气泡的聚合，有利于泡沫的稳定性。pH为13时，OVT的表面压力较高（13.579 mN/m），有利于泡沫的形成。此外，界面性质是OVT浓度的函数。结果表明，OVT的界面行为受pH和浓度的影响。
The structure of proteins, which is tightly related to their interfacial behaviors, is strongly affected by environmental conditions. The objectives of the present study were to explore the effects of pH on particle size, zeta potential, surface hydrophobicity and -SH of ovotransferrin (OVT), and then compare the interfacial behaviors of OVT at various pHs. The results showed that the zeta potential changed from +10.24 to -18.83 mV over a pH range from 3 to 13. The particle size initially increased and then decreased. In addition, more -SH were exposed and the hydrophobicity of the OVT was enhanced when the pH level deviated from pH 6.5. Compared with OVT at other pH values, at pH 5, OVT had a higher penetration and rearrangement rate, which resulted in it more rapidly forming a high elastin membrane (dilatation viscous modulus, dilatation elastic modulus were 46.32, 16.33 mN/m respectively) at the interface. The high elastic interfacial adsorption layer can prevent the polymerization of bubbles, which is beneficial to the stability of the foam. At pH 13, OVT had a higher surface pressure (13.579 mN/m), which was conducive to the formation of foams. Also, the interfacial properties were a function of the OVT concentration. The data showed that the interfacial behaviors of OVT were influenced by pH and concentration.