Lysozyme is an inducible marker of macrophage activation in murine tissues as demonstrated by in situ hybridization.
Lysozyme is an inducible marker of macrophage activation in murine tissues as demonstrated by in situ hybridization.
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DOI：
10.1084/jem.174.5.1049
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发表时间：
1991-11-01
期刊：
影响因子：
--
通讯作者：
Gordon S
中科院分区：
文献类型：
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作者：
Keshav S;Chung P;Milon G;Gordon S
本研究表明，在体内刺激后，小鼠组织巨噬细胞（M phi）中溶菌酶mRNA原位表达的诱导。大多数组织的静止驻留组织M phi不包含足够的溶菌酶mRNA，以通过使用35S标记的RNA探针进行原位杂交来检测。然而，在卡介苗或约氏疟原虫感染后，聚集到肝脏和脾脏的M phi与溶菌酶探针强烈杂交。感染后24小时内，脾脏边缘区的细胞开始产生溶菌酶mRNA。这种反应也由非感染性因子（静脉注射绵羊红细胞）引起，可能是早期吞噬作用的结果。感染后期，脾脏红色和白色髓中的其他细胞以及肝脏肉芽肿中的细胞变得溶菌酶阳性。枯否细胞很少是溶菌酶阳性。在感染期间，肝肉芽肿中的溶菌酶mRNA水平保持相对恒定，并且大多数肉芽肿细胞产生溶菌酶。这与肿瘤坏死因子α（TNF α）mRNA形成对比，后者由肉芽肿中较少的细胞产生，并且可以通过脂多糖给药大量诱导。因此，先前被认为是M phi的组成性功能的溶菌酶的产生是体内M phi活化的指标，其中免疫特异性和非特异性刺激都刺激占据离散解剖位置的细胞亚群中高水平的溶菌酶产生。
This study demonstrates the induction of lysozyme mRNA expression in situ in tissue macrophages (M phi) of mice following in vivo stimulation. The resting resident tissue M phi of most tissues do not contain enough lysozyme mRNA to be detected by in situ hybridization using 35S-labeled RNA probes. Following Bacille Calmette Guerin or Plasmodium yoelli infection, however, M phi recruited to liver and spleen hybridize strongly to the lysozyme probe. Within 24 h of infection, cells found in the marginal zone of the spleen begin to produce lysozyme mRNA. This response is also evoked by a noninfectious agent (intravenously injected sheep erythrocytes), and is possibly the result of an early phagocytic interaction. Later in the infection, other cells in the red and white pulp of the spleen, and cells in granulomas in the liver, become lysozyme-positive. Kupffer cells are rarely lysozyme-positive. Lysozyme mRNA levels in liver granulomas remain relatively constant during the infection, and lysozyme is produced by most granuloma cells. This contrasts with tumor necrosis factor alpha (TNF alpha) mRNA, which is produced by fewer cells in the granuloma, and which can be massively induced by lipopolysaccharide administration. The production of lysozyme, previously considered a constitutive function of M phi, is therefore an indicator of M phi activation in vivo, where immunologically specific and nonspecific stimuli both stimulate lysozyme production at high levels in subpopulations of cells occupying discrete anatomical locations.