Polysome-like structures in the chromatoid body of rat spermatids
Polysome-like structures in the chromatoid body of rat spermatids
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DOI：
10.1007/s004410051027
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发表时间：
1998-03-01
影响因子：
3.6
通讯作者：
Burzio, LO
中科院分区：
文献类型：
--
作者：
Figueroa, J;Burzio, LO
建立了一种从40日龄大鼠睾丸中分离类染色体的方法。电子显微镜分析表明，约70%的分离的细胞器是类染色体体，而其余的结构对应于致密体，可能卫星。分离的细胞器的负染色显示在约20%的拟染色体体中存在多核糖体样结构，这表明多核糖体不是由于细胞质多核糖体的污染。此外，RNA酶-金染色证实了在拟染色体体的基质中存在RNA，从细胞器中提取的RNA的初步电泳分析揭示了一个复杂的RNA群体的存在，包括5.8和5S核糖体RNA，但没有tRNA。
A procedure for isolating the chromatoid body from the testis of 40-day-old rats was developed. Electron-microscopical analysis indicated that about 70% of the isolated organelles were chromatoid bodies, while the remaining structures corresponded to dense bodies and probably to satellites. Negative staining of the isolated organelles revealed the presence of polysome-like structures in about 20% of the chromatoid bodies suggesting that the polysomes were not due to contamination with cytoplasmic polysomes, Moreover, the presence of RNA in the stroma of the chromatoid body was confirmed by RNAse-gold staining, Preliminary electrophoretic analysis of the RNA extracted from the organelles revealed the presence of a complex population of RNAs including 5.8 and 5 S ribosomal RNAs but no tRNA.