Complete genome analysis and molecular characterization of Usutu virus that emerged in Austria in 2001 -: Comparison with the South African strain SAAR-1776 and other flaviviruses
Complete genome analysis and molecular characterization of Usutu virus that emerged in Austria in 2001 -: Comparison with the South African strain SAAR-1776 and other flaviviruses
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DOI：
10.1016/j.virol.2004.08.005
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发表时间：
2004-10-25
期刊：
影响因子：
3.7
通讯作者：
Nowotny, N
中科院分区：
文献类型：
--
作者：
Bakonyi, T;Gould, EA;Nowotny, N
在这里，我们描述了完整的基因组序列的两个菌株的ESTU病毒（USUV），蚊子传播的成员属黄病毒在日本脑炎病毒（JEV）血清组。2001年在奥地利检测到USUV，导致黑鸟的高死亡率;参考菌株（SAAR-1776）于1958年从南非的蚊子中分离出来，从未与鸟类死亡有关。奥地利和南非分离株显示97%的核苷酸和99%的氨基酸相同性。构建了系统发育树，显示了USUV与黄病毒属其他成员的遗传关系。当将USUV与其他JEV血清群病毒比较时，最接近的谱系是墨累谷脑炎病毒（nt：73%，aa：82%），其次是JEV（nt：71%，aa：81%）和西尼罗病毒（nt：68%，aa：75%）。基因组的比较表明，保守的结构元件和推定的酶基序在两个USUV株和JEV血清群是同源的。决定严重的临床症状所造成的奥地利USUV株欧亚黑鸟的因素进行了讨论。我们还提供了一个可能的解释的起源和分散的USUV，JEV，和MVEV出非洲。(C)2004爱思唯尔公司All rights reserved.
Here we describe the complete genome sequences of two strains of Usutu virus (USUV), a mosquito-borne member of the genus Flavivirus in the Japanese encephalitis virus (JEV) serogroup. USUV was detected in Austria in 2001 causing a high mortality rate in blackbirds; the reference strain (SAAR-1776) was isolated in 1958 from mosquitoes in South Africa and has never been associated with avian mortality. The Austrian and South African isolates exhibited 97% nucleotide and 99% amino acid identity. Phylogenetic trees were constructed displaying the genetic relationships of USUV with other members of the genus Flavivirus. When comparing USUV with other JEV serogroup viruses, the closest lineage was Murray Valley encephalitis virus (nt: 73%, aa: 82%) followed by JEV (nt: 71%, aa: 81%) and West Nile virus (nt: 68%, aa: 75%). Comparison of the genomes showed that the conserved structural elements and putative enzyme motifs were homologous in the two USUV strains and the JEV serogroup. The factors that determine the severe clinical symptoms caused by the Austrian USUV strain in Eurasian blackbirds are discussed. We also offer a possible explanation for the origins and dispersal of USUV, JEV, and MVEV out of Africa. (C) 2004 Elsevier Inc. All rights reserved.