Proposal of Histophilus somni gen. nov., sp nov for the three species incertae sedis 'Haemophilus somnus', 'Haemophilus agni' and 'Histophilus ovis'
Proposal of Histophilus somni gen. nov., sp nov for the three species incertae sedis 'Haemophilus somnus', 'Haemophilus agni' and 'Histophilus ovis'
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DOI：
10.1099/ijs.0.02637-0
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发表时间：
2003-09-01
影响因子：
2.8
通讯作者：
Mutters, R
中科院分区：
文献类型：
--
作者：
Angen, O;Ahrens, P;Mutters, R
早前的调查显示，‘嗜血杆菌’、‘烈性嗜血杆菌’和‘绵羊组织嗜血杆菌’代表同一物种。在本次调查中，通过对以前用DNA-DNA杂交方法研究过的菌株的16S rRNA和rpoB基因进行测序，进一步探讨了该物种的分类地位。这些结果清楚地支持将该种归入巴氏杆菌科中的一个新属。索马里组织嗜血杆菌的表型分离。Nov.，sp.11月对于大多数菌株来说，来自该家族其他成员的基因可能是基于嗜毛症、淡黄色色素沉着和吲哚产生。然而，由于表型差异，物种描述中包括使用基于16S rRNA基因的物种特异性PCR检测。这是因为16S rRNA基因在物种内具有很高的序列相似性，并且与该科内任何其他分类单元的最高序列相似性为93.4%。模式菌株8025(T)=ATCC 43625(T)CCUG 36157(T)是在美国从一只患有血栓栓塞性脑膜脑炎的牛脑中分离到的。
Earlier investigations have shown that 'Haemophilus somnus','Haemophilus agni' and' Histophilus ovis' represent the same species. In the present investigation, the taxonomic position of this species is explored further by sequencing the 16S rRNA and rpoB genes of strains that were investigated previously by DNA-DNA hybridization. These results clearly support the allocation of this species to a novel genus within the family Pasteurellaceae. The phenotypic separation of Histophilus somni gen. nov., sp. nov. from other members of the family can, for most strains, be based on capnophilia, yellowish pigmentation and indole production. However, due to phenotypic variation, the use of a species-specific PCR test based on the 16S rRNA gene is included in the species description. This is justified by the high sequence similarity of the 16S rRNA gene within the species and the fact that the highest sequence similarity to any other taxon within the family is 93.4%. The type strain, 8025(T) = ATCC 43625(T) CCUG 36157(T), was isolated in the USA from a bovine brain with lesions of thromboembolic meningoencephalitis.