A cost-effective microbial fuel cell to detect and select for photosynthetic electrogenic activity in algae and cyanobacteria
A cost-effective microbial fuel cell to detect and select for photosynthetic electrogenic activity in algae and cyanobacteria
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DOI：
10.1007/s10811-013-0051-2
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发表时间：
2014-02-01
影响因子：
3.3
通讯作者：
Packer, Michael A.
中科院分区：
文献类型：
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作者：
Luimstra, Veerle M.;Kennedy, Sophie-Jean;Packer, Michael A.
这项工作描述了一种易于构建的，具有成本效益的光合微生物燃料电池设计的发展具有高度可重复的电化学特性，可用于筛选藻类和蓝藻光合产电活性。它特别适用于附着在表面的底栖品种。电池的阳极室使用一次性聚苯乙烯样品瓶（瓶），其具有易于应用的碳涂层。这些腔室可用于生长光合微生物，而无需阴极进行电化学表征，或具有阴极和施加的负载以提供产电选择性压力。证明了在各种条件下筛选、分离和分析光合产电微生物的设计的实用性。来自新西兰和南极的几个属的底栖蓝细菌被证明是产电的，包括伪链藻属、细鳞藻属、色球藻属、席藻属、微鞘藻属、念珠藻属和席藻属。分离并鉴定了一株真核生物Paulschulzia pseudovolvox（绿藻科）底栖菌株，该菌株具有很好的产电特性。
This work describes the development of an easily constructed, cost-effective photosynthetic microbial fuel cell design with highly reproducible electrochemical characteristics that can be used to screen algae and cyanobacteria for photosynthetic electrogenic activity. It is especially suitable for benthic varieties, those that attach to surfaces. The anode chamber of the cell uses disposable polystyrene sample bottles (pottles) with a simple-to-apply carbon coating. These chambers can be used to grow photosynthetic microorganisms without a cathode for electrochemical characterization or with a cathode and load applied to provide electrogenic selective pressure. The utility of the design for screening, isolating and analysing photosynthetic electrogenic microorganisms under a wide variety of conditions is demonstrated. Several genera of benthic cyanobacteria from both New Zealand and Antarctica were shown to be electrogenic including Pseudanabaena, Leptolyngbya, Chroocococales, Phormidesmis, Microcoleus, Nostoc and Phormidium. A benthic strain of the eukaryote Paulschulzia pseudovolvox (Chlorophyceae) was isolated and identified, which had very good electrogenic qualities.