Anti-Ace monoclonal antibody reduces Enterococcus faecalis aortic valve infection in a rat infective endocarditis model.
Anti-Ace monoclonal antibody reduces Enterococcus faecalis aortic valve infection in a rat infective endocarditis model.
复制标题
抗 Ace 单克隆抗体可减少大鼠感染性心内膜炎模型中粪肠球菌主动脉瓣感染。
DOI：
10.1093/femspd/fty084
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发表时间：
2018
影响因子：
3.3
通讯作者：
Murray,BarbaraE
中科院分区：
文献类型：
--
作者：
Singh,KavindraV;Pinkston,KennethL;Gao,Peng;Harvey,BarrettR;Murray,BarbaraE
Ace（粪肠球菌胶原粘附素）是一种细胞壁锚定蛋白，在大鼠感染性心内膜炎（IE）模型中条件性表达，对毒力非常重要。以前，我们表明，大鼠免疫的胶原蛋白结合域的ACE（域A），或管理抗ACE域A多克隆抗体，是不太容易受到E。粪便心内膜炎比假免疫对照。在这项工作中，我们证明了一个亚纳摩尔的单克隆抗体（mAb），抗Ace mAb 70，显着减少E。在体外粘附试验中，抗Ace mAb 70预处理显著降低E.主动脉瓣的粪便感染。抗Ace单克隆抗体在大鼠模型中抗IE的有效性表明，它可能是一种有益的被动保护剂。粪便感染。
Ace (Adhesin to collagen fromEnterococcus faecalis) is a cell-wall anchored protein that is expressed conditionally and is important for virulence in a rat infective endocarditis (IE) model. Previously, we showed that rats immunized with the collagen binding domain of Ace (domain A), or administered anti-Ace domain A polyclonal antibody, were less susceptible toE. faecalisendocarditis than sham-immunized controls. In this work, we demonstrated that a sub nanomolar monoclonal antibody (mAb), anti-Ace mAb70, significantly diminishedE. faecalisbinding to ECM collagen IV inin vitroadherence assays and that, in the endocarditis model, anti-Ace mAb70pre-treatment significantly reducedE. faecalisinfection of aortic valves. The effectiveness of anti-Ace mAb against IE in the rat model suggests it might serve as a beneficial agent for passive protection againstE. faecalisinfections.