Vitamin D3 analogs stimulate hair growth in nude mice
Vitamin D3 analogs stimulate hair growth in nude mice
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DOI：
10.1210/en.2002-220118
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发表时间：
2002-11-01
期刊：
影响因子：
4.8
通讯作者：
Koeffler, HP
中科院分区：
文献类型：
--
作者：
Vegesna, V;O'Kelly, J;Koeffler, HP
维生素D-3的活性形式可以通过诱导终末分化来调节表皮角化;缺乏维生素D受体的小鼠表现出导致产后脱发的缺陷。这些观察结果暗示维生素D-3途径在头发生长的调节。我们测试了1，25二羟维生素D-3及其合成类似物刺激表现出先天性脱发的biege/nude/xid（BNX）nu/nu（裸）小鼠毛发生长的能力。用不同的维生素D-3类似物处理裸鼠，剂量是我们以前发现的最高剂量，不会引起毒性（高钙血症）。监测小鼠的毛发生长，并根据定义的量表进行评分。取皮肤样品进行毛囊的组织学观察和RNA和蛋白质的提取。维生素D-3类似物显着刺激裸鼠的毛发生长，尽管亲本1，25二羟维生素D-3没有效果。毛发生长呈周期性，伴随着正常毛囊的形成和某些角蛋白（Ha 7、Ha 8和Hb 3）表达的增加。维生素D-3类似物似乎作用于角质形成细胞，启动毛囊循环，刺激小鼠的毛发生长，否则不会生长毛发。
The active form of vitamin D-3 can regulate epidermal keratinization by inducing terminal differentiation; and mice lacking the vitamin D receptor display defects leading to postnatal alopecia. These observations implicate the vitamin D-3 pathway in regulation of hair growth. We tested the ability of 1,25 dihydroxyvitamin D-3 and its synthetic analogs to stimulate hair growth in biege/nude/xid (BNX) nu/nu (nude) mice exhibiting congenital alopecia. Nude mice were treated with different vitamin D-3 analogs at doses that we had previously found to be the highest dose without inducing toxicity (hypercalcemia). The mice were monitored for hair growth and were scored according to a defined scale. Skin samples were taken for histological observation of hair follicles and for extraction of RNA and protein. Vitamin D-3 analogs dramatically stimulated the hair growth of nude mice, although parental 1,25 dihydroxyvitamin D-3 had no effect. Hair growth occurred in a cyclical pattern, accompanied by formation of normal hair follicles and increased expression of certain keratins (Ha7, Ha8, and Hb3). Vitamin D-3 analogs seem to act on keratinocytes to initiate hair follicle cycling and stimulate hair growth in mice that otherwise do not grow hair.