Cestrum nocturnum Flower Extracts Attenuate Proliferation and Induce Apoptosis in Malignant Cells through Inducing DNA Damage and Inhibiting Topoisomerase II Activity.
Cestrum nocturnum Flower Extracts Attenuate Proliferation and Induce Apoptosis in Malignant Cells through Inducing DNA Damage and Inhibiting Topoisomerase II Activity.
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Cestrum nocturnum 花提取物通过诱导 DNA 损伤和抑制拓扑异构酶 II 活性来减弱恶性肿瘤细胞的增殖并诱导细胞凋亡
DOI：
10.1155/2017/1456786
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Zhong ZG
中科院分区：
文献类型：
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作者：
Wu DP;Lin TY;Lv JY;Chen WY;Bai LR;Zhou Y;Huang JL;Zhong ZG
大多数现有的化疗药物都有很多副作用。中草药用于药物和食疗已有数千年的历史，其疗效更佳，副作用更少。 Cestrum nocturnum (CN) 在中国几个世纪以来一直被用来治疗消化系统疾病。我们前期的研究首先证明了从CN花中分离出来的正丁醇部分对恶性细胞的增殖有抑制作用。然而，CN花中正丁醇部分的抗增殖作用的组分及相关机制仍不清楚。因此，在本研究中，我们从 CN 花的正丁醇部分中提取了组分 C4 和 C5，并研究了它们的免疫毒性和抗肿瘤活性。结果发现，C4和C5级分在体外对癌细胞系表现出极大的细胞毒性，但对T和B淋巴细胞具有较低的免疫毒性。测试的组分还通过诱导 DNA 损伤和抑制拓扑异构酶 II 松弛活性，减弱 Bel-7404 细胞 G0/G1 和 G2/M 期的增殖并诱导细胞凋亡。这些结果表明，C4 和 C5 级分由于其显着的抗肿瘤作用和低免疫毒性，可能代表潜在抗肿瘤药物的重要来源。
Most of the existing chemotherapeutic drugs have plenty of side effects. Chinese herbal medicine has been used for pharmaceutical and dietary therapy for thousands of years with more effective and fewer side effects. Cestrum nocturnum (CN) has long been used to treat digestive diseases for centuries in China. Our previous study first proved that the n-butanol part isolated from the flowers of CN produced an inhibitory effect on the proliferation of malignant cells. However, the fractions responsible for the antiproliferation effect of n-butanol part from CN flowers and related mechanisms remain unknown. Thus, in this study, we extracted fractions C4 and C5 from n-butanol part of CN flowers and investigated their immune toxicity and antitumor activities. It was found that fractions C4 and C5 exhibited great cytotoxicity to cancer cell lines but had low immune toxicity towards T and B lymphocytes in vitro. The tested fractions also attenuated proliferation and induced apoptosis at G0/G1 and G2/M phases in Bel-7404 cells through inducing DNA damage and inhibiting topoisomerase II relaxation activity. These results suggest that fractions C4 and C5 may represent important sources of potential antitumor agents due to their pronounced antitumor effects and low immune toxicity.