The meiotic bouquet promotes homolog interactions and restricts ectopic recombination in Schizosaccharomyces pombe
The meiotic bouquet promotes homolog interactions and restricts ectopic recombination in Schizosaccharomyces pombe
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DOI：
10.1534/genetics.106.059733
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发表时间：
2006-09-01
期刊：
影响因子：
3.3
通讯作者：
Smith, Gerald R.
中科院分区：
文献类型：
--
作者：
Davis, Luther;Smith, Gerald R.
当细胞进入减数分裂时，染色体结构发生广泛的程序性变化。一个高度保守的变化是端粒聚集在核周围形成“花束”构型。在裂殖酵母裂殖酵母中，花束和相关的核运动促进同源物之间的初始相互作用。我们发现，Bqt2，一个减数分裂特异性蛋白所需的花束形成，需要野生型水平的同源配对和减数分裂等位基因重组。在bqt2 Delta突变体中，基因转换和交换都减少，并表现出负干扰，反映了同源配对减少。虽然花束和核运动都促进配对，但只有花束限制异位重组（分散的重复DNA之间的重组）。我们讨论的机制，花束可能会防止有害的易位限制异位重组。
Chromosome architecture undergoes extensive, programmed changes as cells enter meiosis. A highly conserved change is the clustering of telomeres at the nuclear periphery to form the "bouquet" configuration. In the fission yeast Schizosaccharomyces pombe the bouquet and associated nuclear movement facilitate initial interactions between homologs. We show that Bqt2, a meiosis-specific protein required for bouquet formation, is required for wild-type levels of homolog pairing and meiotic allelic recombination. Both gene conversion and crossing over are reduced and exhibit negative interference in bqt2 Delta mutants, reflecting reduced homolog pairing. While both the bouquet and nuclear movement promote pairing, only the bouquet restricts ectopic recombination (that between dispersed repetitive DNA). We discuss mechanisms by which the bouquet may prevent deleterious translocations by restricting ectopic recombination.