SEROTYPING FOR HOMOTRANSPLANTATION .18. REFINEMENT OF MICRODROPLET LYMPHOCYTE CYTOTOXICITY TEST
SEROTYPING FOR HOMOTRANSPLANTATION .18. REFINEMENT OF MICRODROPLET LYMPHOCYTE CYTOTOXICITY TEST
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DOI：
10.1097/00007890-196811000-00006
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发表时间：
1968-01-01
期刊：
影响因子：
6.2
通讯作者：
TERASAKI, PI
中科院分区：
文献类型：
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作者：
MITTAL, KK;MICKEY, MR;TERASAKI, PI
微滴淋巴细胞毒性试验进行了彻底的检查，以增加试验的可重复性。通过在技术中引入某些修改，大系列测试中的差异率从本研究开始时的5.16%降低到目前的0.95%。研究了与淋巴细胞分离、抗血清处理、抗血清质量、补体量、孵育温度、孵育时间、固定反应和读取反应有关的变量。所建立的方法具有重复性好、操作简单、易于大规模应用的特点。
The microdroplet lymphocyte cytotoxicity test was examined thoroughly in an effort to increase the reproducibility of the test. The discrepancy rate in a large series of tests was reduced from 5.16% at the start of this study to the present 0.95% by introducing certain modifications in the technique. Variables connected with the isolation of lymphocytes, handling of antisera, quality of antisera, amount of complement, incubation temperature, duration of incubation, fixing of reactions, and reading of reactions were studied. The method which has resulted appears to be reproducible, simple, and readily usable on a large scale.