Gene-Environment Interactions at Nucleotide Resolution
Gene-Environment Interactions at Nucleotide Resolution
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DOI：
10.1371/journal.pgen.1001144
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发表时间：
2010-09-01
期刊：
影响因子：
4.5
通讯作者：
Cohen, Barak
中科院分区：
文献类型：
--
作者：
Gerke, Justin;Lorenz, Kim;Cohen, Barak
基因和环境之间的相互作用是表型变异的常见来源。为了在单核苷酸分辨率下表征遗传和环境之间的相互作用，我们量化了控制酵母产孢率的四个数量性状核苷酸(QTN)的遗传和环境相互作用。我们首先构建了一组菌株，这些菌株在两个不同的遗传背景中总共携带了4种QTN基因的所有32种可能的组合。然后，我们测量了这32个菌株在8个受控环境中的产孢率。这个数据集表明，产孢率的变化很大程度上是由遗传和环境相互作用决定的。我们找到了QTN：环境、QTN：背景和环境：背景交互的明显示例。然而，我们没有发现在整个数据集中一致发生的QTN：QTN交互。相反，QTN之间的相互作用只在特定的环境和遗传背景组合下发生。因此，当我们将整个数据集作为一个整体考虑时，在一个背景和环境中看起来可能是QTN：QTN的交互作用变成了更复杂的QTN：QTN：环境：背景交互作用。因此，一组QTN等位基因的表型影响不能仅从基因型来预测。相反，我们的结果表明，QTN的影响及其相互作用与遗传背景和环境变异都有着千丝万缕的联系。
Interactions among genes and the environment are a common source of phenotypic variation. To characterize the interplay between genetics and the environment at single nucleotide resolution, we quantified the genetic and environmental interactions of four quantitative trait nucleotides (QTN) that govern yeast sporulation efficiency. We first constructed a panel of strains that together carry all 32 possible combinations of the 4 QTN genotypes in 2 distinct genetic backgrounds. We then measured the sporulation efficiencies of these 32 strains across 8 controlled environments. This dataset shows that variation in sporulation efficiency is shaped largely by genetic and environmental interactions. We find clear examples of QTN:environment, QTN:background, and environment:background interactions. However, we find no QTN:QTN interactions that occur consistently across the entire dataset. Instead, interactions between QTN only occur under specific combinations of environment and genetic background. Thus, what might appear to be a QTN:QTN interaction in one background and environment becomes a more complex QTN:QTN:environment:background interaction when we consider the entire dataset as a whole. As a result, the phenotypic impact of a set of QTN alleles cannot be predicted from genotype alone. Our results instead demonstrate that the effects of QTN and their interactions are inextricably linked both to genetic background and to environmental variation.