Adaptor protein complex-4 (AP-4) is expressed in the central nervous system neurons and interacts with glutamate receptor δ2
Adaptor protein complex-4 (AP-4) is expressed in the central nervous system neurons and interacts with glutamate receptor δ2
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DOI：
10.1016/s1044-7431(03)00164-7
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发表时间：
2003-10-01
影响因子：
3.5
通讯作者：
Yano, R
中科院分区：
文献类型：
--
作者：
Yap, CC;Murate, M;Yano, R
离子通道和受体定位于特定的突触后位点，介导神经传递。突触后受体聚集已被广泛研究；然而，细胞内受体转运到其特定目的地的分子机制尚不清楚。在本研究中，我们发现谷氨酸受体delta2直接与AP-4相互作用，AP-4是一种新发现的介导哺乳动物细胞中蛋白质分选的衔接蛋白复合物-4。AP-4的mu4亚基与delta2 c端之间的相互作用涉及多个氨基酸序列基序，而不是经典的基于酪氨酸的信号。AP-4复合物在大脑的许多区域普遍表达，定位于细胞体和神经元树突的高尔基样结构上。此外，mu4的过表达大大改变了delta2在异源细胞中的分布模式。这些结果表明AP-4可能参与了大脑中delta - 2的运输。(C) 2003 Elsevier Science（美国）版权所有。
Ion channels and receptors are targeted and localized at specific postsynaptic sites to mediate neurotransmission. Receptors clustering at postsynaptic sites has been extensively studied; however, the molecular mechanisms underlying intracellular trafficking of receptors to their specific destinations remain unclear. In the present study, we found that glutamate receptor delta2 interacted directly with AP-4, a newly identified adaptor protein complex-4 that mediates protein sorting in mammalian cells. The interaction between mu4 subunit of AP-4 and the delta2 C-terminal involved multiple amino acid sequence motifs other than the classical tyrosine-based signals. AP-4 complex is expressed ubiquitously in many regions of brain, with localization on the Golgi-like structures in the cell bodies and dendrites of neurons. In addition, overexpression of mu4 substantially altered the distribution pattern of delta2 in heterologous cells. These results suggest a potential involvement of AP-4 in the trafficking of delta2 in the brain. (C) 2003 Elsevier Science (USA). All rights reserved.