Histopathological Evaluation of the Efficacy of Antifungals for Experimental Trichosporon Bloodstream Infection
Histopathological Evaluation of the Efficacy of Antifungals for Experimental Trichosporon Bloodstream Infection
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DOI：
10.7883/yoken.66.133
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发表时间：
2013-03-01
影响因子：
2.2
通讯作者：
Shibuya, Kazutoshi
中科院分区：
文献类型：
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作者：
Sasai, Daisuke;Okubo, Yoichiro;Shibuya, Kazutoshi
通过组织病理学检查和感染小鼠肾脏活细胞计数来评价多烯大环内酯类药物治疗实验性丝孢酵母血流感染的疗效。感染后第5天的活细胞计数证实，脂质体阿替霉素B（L-AMB）比氟康唑（FLC）对感染唑类耐药丝孢酵母菌株的小鼠更有效。组织学检查显示，L-AMB的管理诱导从唑敏感和耐药菌株感染引起的急性化脓性炎症转化为慢性和消退形式，而FLC未能将唑耐药菌株诱导的急性炎症转化为消退形式。我们的研究结果表明，多烯大环内酯类可作为一种替代疗法的唑类耐药菌株的丝孢酵母菌感染和组织病理学评价是有用的阐明实验性丝孢酵母菌感染的病理生理学。
The efficacy of polyene macrolides to treat experimental Trichosporon bloodstream infection was evaluated by histopathological examination and viable cell counts in the kidneys of infected mice. Viable cell counts on the 5th day after infection confirmed that liposomal amphotericin B (L-AMB) is a more effective treatment than fluconazole (FLC) for mice infected with an azole-resistant strain of Trichosporon. Histological examination revealed that the administration of L-AMB induced a transformation from acute purulent inflammation caused by both azole-susceptible and -resistant strain infections to a chronic and subsiding form, whereas FLC failed to convert the acute inflammation induced by the azole-resistant strain to a subsiding form. Our results demonstrate that polyene macrolides can be used as an alternative therapy for infection of azole-resistant strains of Trichosporon and that histopathological evaluation is useful for elucidating the pathophysiology of an experimental Trichosporon infection.