Exposure to 1-bromopropane causes ovarian dysfunction in rats
Exposure to 1-bromopropane causes ovarian dysfunction in rats
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DOI：
10.1093/toxsci/71.1.96
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发表时间：
2003-01-01
影响因子：
3.8
通讯作者：
Takeuchi, Y
中科院分区：
文献类型：
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作者：
Yamada, T;Ichihara, G;Takeuchi, Y
尽管1-溴丙烷已在化学和电子工业中用作消耗臭氧层溶剂的替代品，但其对女性生殖器官的毒性尚未完全阐明。本实验的目的是确定 1-溴丙烷对雌性大鼠生殖功能的影响。将四十只雌性 Wistar 大鼠分为四个相等的组。每组每天暴露于 0、200、400 或 800 ppm 的 1-溴丙烷，每天八小时。暴露 7 周后，800 ppm 组的所有大鼠均病情严重，并在第 8 周处死。其他剂量组暴露 12 周。在 800 ppm 组中，但在其他两个暴露组中，在开始暴露后 2 至 7 周的每个时间点，体重均显着低于对照组。阴道涂片测试显示，400 ppm 和 800 ppm 组的不规则动情周期数量显着增加，且动情间期延长。卵巢的组织病理学检查显示，400 ppm 组中正常窦卵泡数量呈剂量依赖性显着减少，正常生长卵泡数量也减少。与对照组相比，任何组的 LH 或 FSH 血浆浓度均未发现显着变化。我们的结果表明，1-溴丙烷可在非妊娠雌性大鼠中诱导剂量依赖性卵巢功能障碍，并与卵泡生长过程破坏相关。
Although 1-bromopropane has been used in chemical and electronic industries as an alternative to ozone layer-depleting solvents, its toxicity on female reproductive organs has not been fully elucidated. The aim of this experiment was to determine the effect of 1-bromopropane on female reproductive function in rats. Forty female Wistar rats were divided into four equal groups. Each group was exposed daily to 0, 200, 400, or 800 ppm of 1-bromopropane for eight h a day. After exposure for 7 weeks, all rats in the 800-ppm group became seriously ill and were sacrificed during the 8th week. The other dose groups were exposed for 12 weeks. In the 800-ppm group, but not in the other two exposed groups, body weight was significantly less than the control at each time point from 2 to 7 weeks after the beginning of exposure. Tests of vaginal smears showed a significant increase in the number of irregular estrous cycles with extended diestrus in the 400- and 800-ppm groups. Histopathological examination of the ovary showed a significant dose-dependent reduction of the number of normal antral follicles and a decrease in the number of normal growing follicles in the 400-ppm group. No significant change was found in plasma concentrations of LH or FSH in any group when compared with the control. Our results indicate that 1-bromopropane can induce a dose-dependent ovarian dysfunction in nonpregnant female rats associated with disruption in follicular growth process.