Complete chloroplast genome and phylogenetic analysis of Anemone shikokiana
Complete chloroplast genome and phylogenetic analysis of Anemone shikokiana
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DOI：
10.1007/s11033-022-08204-4
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发表时间：
2022-12
影响因子：
2.8
通讯作者：
K. An;Chunxia Zhou;Lei Feng;Qing Zhang;Yujuan Pang;Lixia Li;F. Bian
中科院分区：
文献类型：
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作者：
K. An;Chunxia Zhou;Lei Feng;Qing Zhang;Yujuan Pang;Lixia Li;F. Bian
研究背景四季银莲花Anemone shikokiana（Makino）Makino间断分布于中国山东半岛和日本四国岛，为珍稀濒危植物。为了提供遗传信息和了解其发育过程，我们对A.方法和结果获得了A. shikokiana cp基因组全序列。本研究构建了shikokiana。结果表明，A. shikokiana具有典型的四分环，全长159，286bp。共鉴定出111个独特的基因，包括78个蛋白质编码基因、29个tRNA编码基因和4个rRNA编码基因。共发现37个长重复序列和67个微卫星。A.将shikokiana与Genbank数据库中的11个Anemonecp基因组进行了比较。我们发现不同基因组之间存在一定的差异，特别是在LSC和SSC区域，并确定了asycf 1、ndhE、ndhD、ndhF-trnL、ndhA和ndhF等区域为潜在的分子标记。系统发育分析结果表明，A. narcissiflora是A.结论该结果填补了我国南方红豆杉cp基因组序列信息的差距。shikokiana，为进一步探讨A. shikokianain未来的研究。为银莲花属植物的分子鉴定和系统发育研究提供了宝贵的遗传资源。
BackgroundAnemone shikokiana(Makino) Makino, disjunctly distributed in Shandong Peninsula of China and Shikoku Island of Japan, is a rare and endangered species. To provide genetic information and understand its phylogeny, we conducted research on the chloroplast (cp) genome ofA. shikokiana.Methods and resultsThe complete cp genome sequence ofA. shikokianawas constructed in this study. The results showed that the cp genome ofA. shikokianahas a typical quadripartite cyclic with a total length of 159,286 bp. In total, 111 unique genes were identified, including 78 protein-coding genes, 29 tRNA-coding genes and 4 rRNA-coding genes. A total of 37 long repeat sequences and 67 microsatellites were found in this cp genome. The cp genome ofA. shikokianawas compared with eleven otherAnemonecp genomes available from the Genbank database. We found some variations among the different genomes, especially in the LSC and SSC regions, and identified some regions as potential molecular markers such asycf1,ndhE,ndhD,ndhF-trnL,ndhAandndhF. The results of phylogenetic analysis suggested thatA. narcissiflorawas the closest relative ofA. shikokiana.ConclusionsThe results filled the gap of cp genome sequence information ofA. shikokiana, laying the foundation to explore the evolutionary relationships ofA. shikokianain future studies. It provided a valuable genetic resource for the molecular identification and phylogenetic study ofAnemone.