Histaminergic neurons of the ventral hippocampus and the baso-lateral amygdala of the rat: functional interaction on memory and learning mechanisms
Histaminergic neurons of the ventral hippocampus and the baso-lateral amygdala of the rat: functional interaction on memory and learning mechanisms
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DOI：
10.1016/s0166-4328(01)00272-8
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发表时间：
2002-01
影响因子：
2.7
通讯作者：
E. O. Alvarez;M. B. Ruarte
中科院分区：
文献类型：
--
作者：
E. O. Alvarez;M. B. Ruarte
在大鼠中研究了当两个大脑结构都受到组胺的化学刺激时，杏仁核和腹侧海马在学习条件性回避反应时功能相互作用的可能性。成年雄性大鼠在乙醚麻醉下将引导插管立体定向植入基底外侧杏仁核和腹侧海马。植入后 72 小时，向大鼠显微注射： 1 μl 盐水溶液到两个结构中（SAL+SAL 组）； 9或90 nmol剂量的组胺进入两个结构（HA+HA组）；将 9 或 90 nmol 组胺注入海马，将盐水注入杏仁核（HA+SAL 组）；将盐水注入海马，并将 9 或 90 nmol 组胺注入杏仁核（SAL+HA 组）。注射后五分钟，大鼠在连续八次试验中接受学习任务，包括当 40 kHz 超声波开启 30 秒时避免对动物的脚施加电击。结果表明，向杏仁核和海马体施加任何剂量的组胺都能显着增加逃避潜伏期并损害学习效率。仅海马体或杏仁核的组胺化学刺激选择性地影响逃避潜伏期。结果表明，在处理学习任务期间，杏仁核和海马体的组胺敏感神经元之间存在功能性相互作用。
The possibility of a functional interaction between the amygdala and the ventral hippocampus on learning of a conditioned avoidance response when both brain structures are chemically stimulated with histamine was studied in rats. Adult male rats were stereotaxically implanted under ether anaesthesia with guide cannulae into the baso-lateral amygdala and the ventral hippocampus. Seventy-two hours after the implant, rats were microinjected with: 1 μl saline solution into both structures (SAL+SAL group); 9 or 90 nmol doses of histamine into both structures (HA+HA groups); 9 or 90 nmol histamine into the hippocampus and saline into the amygdala (HA+SAL groups); saline into the hippocampus and 9 or 90 nmol histamine into the amygdala (SAL+HA groups). Five minutes following the injection, rats were subjected to a learning task in eight consecutive trials, consisting of avoiding an electric shock applied to the feet of the animal when an ultrasonic tone of 40 kHz is on for 30 s. Results show that histamine applied in any dose into both amygdala and hippocampus was able to significantly increase the escape latency and impair the efficacy of learning. Chemical stimulation with histamine of only hippocampus or amygdala affected selectively the escape latency. Results suggest that there is a functional interaction between histamine-sensitive neurons of the amygdala and hippocampus during processing of a learning task.