Stiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation
Stiffness and Membrane Anchor Density Modulate DNA-Nanospring-Induced Vesicle Tubulation
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DOI：
10.1021/acsami.9b05401
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发表时间：
2019-07-03
影响因子：
9.5
通讯作者：
Lin, Chenxiang
中科院分区：
文献类型：
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作者：
Grome, Michael W.;Zhang, Zhao;Lin, Chenxiang
DNA纳米技术提供了一个途径，合理设计的人工组装与明确定义和可调的架构建设。弯曲的DNA纳米结构可以模仿天然的膜变形蛋白质，并配备膜锚定分子，可以在体外复制亚细胞膜重塑事件，如囊泡微管。为了系统地分析DNA纳米结构的结构刚度和膜亲和力如何影响膜重塑结果，在这里，我们构建了具有不同厚度和两亲性肽密度的DNA折纸卷曲，并将它们在脂质体表面上嵌入纳米弹簧。我们发现，适度降低刚度和最大限度地增加膜锚的数量不仅促进膜结合和重塑，而且还导致形成具有更好定义的直径的脂质小管，突出了基于可编程DNA的构建体可控地使膜变形的能力。
DNA nanotechnology provides an avenue for the construction of rationally designed artificial assemblages with well-defined and tunable architectures. Shaped to mimic natural membrane-deforming proteins and equipped with membrane anchoring molecules, curved DNA nanostructures can reproduce subcellular membrane remodeling events such as vesicle tubulation in vitro. To systematically analyze how structural stiffness and membrane affinity of DNA nanostructures affect the membrane remodeling outcome, here we build DNA-origami curls with varying thickness and amphipathic peptide density, and have them polymerize into nanosprings on the surface of liposomes. We find that modestly reducing rigidity and maximizing the number of membrane anchors not only promote membrane binding and remodeling but also lead to the formation of lipid tubules with better defined diameters, highlighting the ability of programmable DNA-based constructs to controllably deform the membrane.