Karyopherin β3:: A new cellular target for the HPV-16 E5 oncoprotein
Karyopherin β3:: A new cellular target for the HPV-16 E5 oncoprotein
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DOI：
10.1016/j.bbrc.2008.04.122
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发表时间：
2008-07-11
影响因子：
3.1
通讯作者：
Suprynowicz, Frank A.
中科院分区：
文献类型：
--
作者：
Krawczyk, Ewa;Hanover, John A.;Suprynowicz, Frank A.
流行病学和实验研究表明，高危型人乳头瘤病毒（HPV）是全球宫颈癌的致病因子，其中一半以上的病例与HPV-16有关。除了HPV-16的E6和E7癌蛋白，最近的证据越来越多地表明HPV-16 E5蛋白（16 E5）是致癌转化的重要介质。由于16 E5没有已知的内在酶活性，其对感染细胞的作用最有可能是通过与各种细胞蛋白质的相互作用和/或其与脂筏的记录关联介导的。在本研究中，我们描述了一种新的细胞靶点，结合到COS细胞和稳定的人宫颈细胞系中的16 E5。该靶标是核转运蛋白β 3，核转运受体家族的成员，在核糖体蛋白的核转运和分泌途径中具有关键作用。(C)2008年爱思唯尔公司All rights reserved.
Epidemiological and experimental studies have shown that high-risk human papillomaviruses (HPVs) are the causative agents of cervical cancer worldwide, and that HPV-16 is associated with more than half of these cases. In addition to the well-characterized E6 and E7 oncoproteins of HPV-16, recent evidence increasingly has implicated the HPV-16 E5 protein (16E5) as an important mediator of oncogenic transformation. Since 16E5 has no known intrinsic enzymatic activity, its effects on infected cells are most likely mediated by interactions with various cellular proteins and/or its documented association with lipid rafts. In the present study, we describe a new cellular target that binds to 16E5 in COS cells and in stable human ectocervical cell lines. This target is karyopherin beta 3, a member of the nuclear import receptor family with critical roles in the nuclear import of ribosomal proteins and in the secretory pathway. (C) 2008 Elsevier Inc. All rights reserved.