Metalloproteinase increases in the injured rat spinal cord
Metalloproteinase increases in the injured rat spinal cord
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DOI：
10.1097/00001756-200011090-00029
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发表时间：
2000-11-09
期刊：
影响因子：
1.7
通讯作者：
Romanic, AM
中科院分区：
文献类型：
--
作者：
de Castro, R;Burns, CL;Romanic, AM
脊髓损伤(SCI)后基质金属蛋白酶MMP9和MMP2表达上调。伤后12~24小时，基质金属蛋白酶-9活性最高，5天时基质金属蛋白酶-2活性升高。酶谱分析检测不到基质金属蛋白酶-3，因此其表达水平最高可达极低水平。脊髓组织金属蛋白酶组织抑制物水平不受损伤的影响，可能与损伤后脊髓组织中基质金属蛋白酶-9和基质金属蛋白酶-2活性升高有关。用一种抗体消融它们表明，脊髓损伤后，浸润性中性粒细胞是基质金属蛋白酶-9活性的主要来源，这表明中性粒细胞在脊髓损伤反应中利用了这种蛋白酶。基质金属蛋白酶-9和基质金属蛋白酶-2可能参与脊髓损伤后细胞外基质的分解。神经报告11：3551-3554(C)2000 Lippincott Williams&Wilkins.
Up-regulation of matrix metalloproteinases MMP-9 and MMP-2 after injury to the spinal cord (SCI) is demonstrated. MMP-9 activity maximized at 12-24 h, and MMP-2 rose at 5 days postinjury. MMP-3 was not detectable by zymographic analysis, so its level of expression was, at most, very low. The level of tissue inhibitor of metalloproteinases in the spinal cord was not altered by injury, perhaps permitting increased MMP-9:and MMP-2 activities in situ. Ablating them with an antibody demonstrated that infiltrating neutrophils were the principal source of MMP-9 activity after spinal cord injury, suggesting that neutrophils utilize that proteinase in responding to spinal cord injury. MMP-9 and MMP-2 probably contribute to breakdown of the extracellular matrix following SCI. NeuroReport 11:3551-3554 (C) 2000 Lippincott Williams & Wilkins.