Canonical Wnt signaling is required for ophthalmic trigeminal placode cell fate determination and maintenance
Canonical Wnt signaling is required for ophthalmic trigeminal placode cell fate determination and maintenance
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DOI：
10.1016/j.ydbio.2007.05.032
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发表时间：
2007-08-15
影响因子：
2.7
通讯作者：
Stark, Michael R.
中科院分区：
文献类型：
--
作者：
Lassiter, Rhonda N. T.;Dude, Carolynn M.;Stark, Michael R.
颅基板是外胚层区域，广泛地贡献于脊椎动物外周感觉神经系统。眼三叉神经（opV）基板的发展，它只产生感觉神经元的三叉神经节的眼叶，是一个有用的模型的感觉神经元的发展。虽然关键的分化过程已经在组织和细胞水平上表征，但控制opV基板发育的信号传导途径还没有。在这里，我们在鸡中测试了经典Writ信号传导途径是否调节opV基板发育。通过将Writ报告基因引入鸡胚头部外胚层，我们表明，在Pax3+ opV基板细胞被诱导表达NO时或之后不久，经典途径在Pax3+ opV基板细胞中是活跃的。以及神经元分化标记物Islet 1、神经丝和NeuN，尽管令人惊讶的是，它导致了神经生成素2在opV基板和外胚层其他地方的上调。然而，激活经典的Writ信号传导途径不足以诱导NO（opV基板的最早特异性标志物）。我们的结论是，典型的Writ信号是必要的正常opV基板的发展，并建议，其他分子的线索，除了Writ信号，以促进细胞走向opV基板的命运。(c)2007爱思唯尔公司All rights reserved.
Cranial placodes are ectodermal regions that contribute extensively to the vertebrate peripheral sensory nervous system. The development of the ophthalmic trigeminal (opV) placode, which gives rise only to sensory neurons of the ophthalmic lobe of the trigeminal ganglion, is a useful model of sensory neuron development. While key differentiation processes have been characterized at the tissue and cellular levels, the signaling pathways governing opV placode development have not. Here we tested in chick whether the canonical Writ signaling pathway regulates opV placode development. By introducing a Writ reporter into embryonic chick head ectoderm, we show that the canonical pathway is active in Pax3+ opV placode cells as, or shortly after, they are induced to express NO. Blocking the canonical Writ pathway resulted in the failure of targeted cells to adopt or maintain an opV fate, as assayed by the expression of various markers including Pax3, FGFR4, Eya2, and the neuronal differentiation markers Islet 1, neurofilament, and NeuN, although, surprisingly, it led to upregulation of Neurogenin2, both in the opV placode and elsewhere in the ectoderm. Activating the canonical Writ signaling pathway, however, was not sufficient to induce NO, the earliest specific marker of the opV placode. We conclude that canonical Writ signaling is necessary for normal opV placode development, and propose that other molecular cues are required in addition to Writ signaling to promote cells toward an opV placode fate. (c) 2007 Elsevier Inc. All rights reserved.