Flower color alteration in Lotus japonicus by modification of the carotenoid biosynthetic pathway
Flower color alteration in Lotus japonicus by modification of the carotenoid biosynthetic pathway
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DOI：
10.1007/s00299-006-0302-7
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发表时间：
2007-01
影响因子：
6.2
通讯作者：
Sakae Suzuki;M. Nishihara;T. Nakatsuka;N. Misawa;I. Ogiwara;S. Yamamura
中科院分区：
文献类型：
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作者：
Sakae Suzuki;M. Nishihara;T. Nakatsuka;N. Misawa;I. Ogiwara;S. Yamamura
To establish a model system for alteration of flower color by carotenoid pigments, we modified the carotenoid biosynthesis pathway ofLotus japonicususing overexpression of thecrtWgene isolated from marine bacteriaAgrobacterium aurantiacumand encoding β-carotene ketolase (4,4′-β-oxygenase) for the production of pink to red color ketocarotenoids. ThecrtWgene with the transit peptide sequence of the pea Rubisco small subunit under the regulation of the CaMV35S promoter was introduced toL. japonicus. In most of the resulting transgenic plants, the color of flower petals changed from original light yellow to deep yellow or orange while otherwise exhibiting normal phenotype. HPLC and TLC analyses revealed that leaves and flower petals of these plants accumulated novel carotenoids, believed to be ketocarotenoids consisting of including astaxanthin, adonixanthin, canthaxanthin and echinenone. Results indicated that modification of the carotenoid biosynthesis pathway is a means of altering flower color in ornamental crops.
