Production of succinic acid by engineered E. coli strains using soybean carbohydrates as feedstock under aerobic fermentation conditions
Production of succinic acid by engineered E. coli strains using soybean carbohydrates as feedstock under aerobic fermentation conditions
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DOI：
10.1016/j.biortech.2012.10.154
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发表时间：
2013-02-01
影响因子：
11.4
通讯作者：
Bennett, George N.
中科院分区：
文献类型：
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作者：
Thakker, Chandresh;San, Ka-Yiu;Bennett, George N.
对含有pRU600和pKK313的大肠杆菌菌株HL2765和HL27659k，在有氧条件下使用从豆粕和大豆可溶物中提取的半乳糖、蔗糖、棉子糖、水苏糖以及这些糖的混合物进行琥珀酸生产的检测。HL2765（pKK313）（pRU600）和HL27659k（pKK313）（pRU600）分别消耗了87 mM和98 mM豆粕提取物中的己糖，并分别产生了83 mM和95 mM琥珀酸。在使用大豆可溶物提取物时，HL2765（pKK313）（pRU600）和HL27659k（pKK313）（pRU600）分别消耗了160 mM和187 mM己糖，并分别产生了158 mM和183 mM琥珀酸。在使用豆粕或大豆可溶物提取物的酸水解产物时，HL2765（pKK313）（pRU600）的琥珀酸产量比HL27659k（pKK313）（pRU600）低。HL27659k（pKK313）（pRU600）使用大豆可溶物水解产物时，获得了最高为312 mM的琥珀酸产量，摩尔产率为0.82 mol/mol己糖。这项研究证明了利用可再生原料大豆中的可溶性碳水化合物作为一种廉价的碳源，通过发酵来生产琥珀酸。（C）2012爱思唯尔有限公司。保留所有权利。
Escherichia coli strains HL2765 and HL27659k harboring pRU600 and pKK313 were examined for succinate production under aerobic conditions using galactose, sucrose, raffinose, stachyose, and mixtures of these sugars extracted from soybean meal and soy solubles. HL2765(pKK313)(pRU600) and HL27659k(pKK313)(pRU600) consumed 87 mM and 98 mM hexose of soybean meal extract and produced 83 mM and 95 mM succinate, respectively. While using soy solubles extract, HL2765(pKK313)(pRU600) and HL27659k(pKK313)(pRU600) consumed 160 mM and 187 mM hexose and produced 158 mM and 183 mM succinate, respectively. Succinate yield of HL2765(pKK313)(pRU600) was low as compared to that of HL27659k(pKK313)(pRU600) while using acid hydrolysate of soybean meal or soy solubles extracts. Maximum succinate production of 312 mM with a molar yield of 0.82 mol/mol hexose was obtained using soy solubles hydrolysate by HL27659k(pKK313)(pRU600). This study demonstrated the use of soluble carbohydrates of the renewable feedstock, soybean as an inexpensive carbon source to produce succinate by fermentation. (C) 2012 Elsevier Ltd. All rights reserved.