Relative stability of ploidy in a marine Synechococcus across various growth conditions.
Relative stability of ploidy in a marine Synechococcus across various growth conditions.
复制标题
海洋聚球藻倍性在各种生长条件下的相对稳定性。
DOI：
10.1111/1758-2229.12614
复制
发表时间：
2018
影响因子：
3.3
通讯作者：
Perez-Sepulveda B
中科院分区：
文献类型：
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作者：
Perez-Sepulveda B
聚球藻属的海洋picocyanobacteria是海洋系统中普遍存在的光养细菌。与这些生物占据了营养贫乏的海洋的大片区域相一致的是，到目前为止研究的大多数海洋藻都是单倍体的，也就是说，只有一个染色体拷贝。唯一的例外是少倍体菌株聚球菌。WH7803，平均有4个染色体拷贝。在这里，我们着手了解资源可用性（通过营养耗尽生长）和物理应激源（紫外线，暴露于低温和高温）在调节该菌株倍性水平中的作用。利用qPCR方法分析了聚球藻的倍性状态，证明了其染色体拷贝数的相对稳定性。WH7803。即使在营养和生理压力下，这种维持寡倍体状态的健壮性表明了一种基本作用，可能是由于长期暴露于氧化应激而促进受损DNA区域的重组，或者通过拥有多个等位基因来增加基因表达的灵活性。
Marine picocyanobacteria of the genusSynechococcusare ubiquitous phototrophs in oceanic systems. Consistent with these organisms occupying vast tracts of the nutrient impoverished ocean, most marineSynechococcusso far studied are monoploid, i.e., contain a single chromosome copy. The exception is the oligoploid strainSynechococcussp. WH7803, which on average possesses around 4 chromosome copies. Here, we set out to understand the role of resource availability (through nutrient deplete growth) and physical stressors (UV, exposure to low and high temperature) in regulating ploidy level in this strain. Using qPCR to assay ploidy status we demonstrate the relative stability of chromosome copy number inSynechococcussp. WH7803. Such robustness in maintaining an oligoploid status even under nutrient and physical stress is indicative of a fundamental role, perhaps facilitating recombination of damaged DNA regions as a result of prolonged exposure to oxidative stress, or allowing added flexibility in gene expression via possessing multiple alleles.