Co-expression of Xylose Transporter and Fructose-Bisphosphate Aldolase Enhances the Utilization of Xylose by Lactococcus lactis IO-1
Co-expression of Xylose Transporter and Fructose-Bisphosphate Aldolase Enhances the Utilization of Xylose by Lactococcus lactis IO-1
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DOI：
10.1007/s12010-022-04168-0
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发表时间：
2022-10
影响因子：
3
通讯作者：
Yejuan Qiu;Zhongyang Qiu;Jun Xia;Xiaoyan Liu;Hanwen Zhang;Yuxiang Yang;Wenyi Hou;Xiangqian Li;Jianlong He
中科院分区：
文献类型：
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作者：
Yejuan Qiu;Zhongyang Qiu;Jun Xia;Xiaoyan Liu;Hanwen Zhang;Yuxiang Yang;Wenyi Hou;Xiangqian Li;Jianlong He
乳酸发酵的原料成本占生产成本的主要部分，这就需要在乳酸生产中探索廉价原料的高效利用。我们比较了木糖转运蛋白（xyylfgh、xylE、araE和xylt）、6-磷酸果糖激酶（pfkA）、果糖二磷酸醛缩酶（fbaA）的同源表达及其在乳酸乳球菌（lactococcus lactisIO-1）中的共表达对以木糖为原料的乳酸生成的影响。我们发现木糖发酵乳酸的产率为l。laactisio -1过表达率最高（14.42%）。在研究的木糖转运蛋白中，XylT在l中具有最强的木糖转运能力。乳酸sio -1，乳酸产率提高10.38%。在代谢通路中，baaorxyln过表达位点附近的基因比远端基因表达量更高。在木糖发酵过程中，共表达baaandxyltl可使乳酸的产率提高27.84%。lactisIO-1。这项工作提出了一种同时增强l木糖代谢途径开始和中途重要基因表达的新策略。乳酸sio -1，可以大大提高靶材产量。图形抽象
The raw material cost of lactic acid fermentation accounts for the main part of the production cost, and this necessitates the exploration of the efficient use of cheap raw materials in lactic acid production. We compared the outcomes of the homologous expressions of xylose transporters (xylFGH,xylE,araE, andxylT), 6-phosphofructokinase (pfkA), fructose-bisphosphate aldolase (fbaA), and their co-expression inLactococcus lactisIO-1 on lactic acid production using xylose as the raw material. We found that the production rate of lactic acid on xylose fermentation byL. lactisIO-1 overexpressingfbaAwas the highest (14.42%). Among the xylose transporters investigated, XylT had the strongest xylose transport capacity inL. lactisIO-1, with an increase in the lactic acid production rate by 10.38%. The genes near the overexpression offbaAorxylTin the metabolic pathway were more upregulated than the distant genes. The co-expression offbaAandxylTincreased the production rate of lactic acid by 27.84% on xylose fermentation byL. lactisIO-1. This work presents a novel strategy for the simultaneous enhancement of the expression of important genes at the beginning and midway of the xylose metabolic pathway ofL. lactisIO-1, which could greatly improve the target production.Graphical abstract